On pancreatic lipase. III. The separation of lipase from its co-enzyme / by O. Rosenheim.

  • Rosenheim, Otto, 1871-1955.
Date:
1910
Catalogue details

Licence: In copyright

Credit: On pancreatic lipase. III. The separation of lipase from its co-enzyme / by O. Rosenheim. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    Journal Physiology, Vol. XL. [From the Proceedings oj the Physiological Society, its co-enzyme. By O. Rosenheim. In tbe preceding communication it was mentioned that a strongly lipoclastic glycerin extract of pancreas was found to be inactive after careful filtration. This holds true for both the diluted and undiluted extract aud agrees with most of the previous statements in literature. Owing to this behaviour the lipoclastic enzyme of the pancreas is now generally considered as an intracellular enzyme. No attempt seems to have been made to examine the fraction of the extract which remains behind on the filter, probably owing to its small amount and its uninviting physical properties. The observation was made that on diluting a glycerin extract of pancreas with water aud allowing the milky fluid to stand, a bulky white precipitate settled, whilst the supernatant fluid became perfectly clear. As the latter was found to be inactive, it was hoped to obtain the active enzyme from the precipitate. To test this point a number of experiments were made on a small scale. Amounts of 5 c.c. of the diluted extract were filtered under pressure through a layer of paper pulp and a perfectly clear filtrate was obtained. Both filtrate and paper pulp with its contents were tested separately with regard to their lipoclastic action, a corresponding amount of unfiltered extract serving as a control. The somewhat surprising fact was found, that the insoluble residue was practically inactive. In order to avoid a possible inhibitory action of the paper pulp, the experiments were repeated by simply effecting the separation of the precipitate by means of the centrifuge. The same result was obtained. On mixing, however, either the paper pulp residue or the centri- fugalised deposit with the clear fluid the two previously inactive constituents of the mixture exerted the same lipoclastic power as the control unfiltered extract. This behaviour is clearly shown in the following experiment. 10 c.c. of glycerin extract were diluted with 40 c.c. water. 5 c.c. of the dilute extract were used for each experiment and allowed to act on 5 c.c. of olive oil emulsion for 18 hours at 37J C. AI