Experimental agglutinative and hemolytic transfusions / Reuben Ottenberg, D. J. Kaliski, and S. S. Friedman.

  • Ottenberg, Reuben, 1882-1959.
Date:
1913
Catalogue details

Licence: In copyright

Credit: Experimental agglutinative and hemolytic transfusions / Reuben Ottenberg, D. J. Kaliski, and S. S. Friedman. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    p. 1 Two years ago the authors determined to find out by experiment what would happen when hemolytic or agg u tinative blood was transfused directly between two ammals of identical species. Although the work had to ' rupted for extraneous reasons over a year ago, and th s account the series of experiments is incomplet^ e resu so far, while not leading to final conclusions, offer so much of interest that it is thought worth while to present them now. Observations on isoagglutination in dogs. — The firstTlifficulty was the choice of animals. The smaller animals such as rats and rabbits, in which the authors have been able to find isoagglutinins, were not available on account ot the difficulty of doing direct transfusions between them. In dogs, the ideal experimental animals, previous workers (like the authors at first) had repeatedly failed to find isoagglu- tinins or isohemolysins. After several failures, however, the technical difficulties, which had previously interfered with the demonstration of such antibodies, were at least partly over- come; and it was then readily demonstrated that isoagglu- tination and isohemolysis occasionally occur between the bloods of apparently normal dogs. The technical difficulty to be overcome was the fragility of dog blood cells, which were found to lake more or less when suspended in salt solution of any strength. Various concen- trations (between 0.6 per cent and two per cent) of sodium chloride were tried with the same result. In the experi- ments, therefore, salt solution was avoided entirely — the ♦From the Biochemical Laboratory of Columbia University at the College of Phy- sicians and Surgeons, and the Pathological Laboratory of Mount Sinai Hospital, under the auspices of the George Crocker Special Research Fund. Received for publication March 21, 1913.