Chromatogram prints [2]

item

Galleries and Reading Room

Library

Shop

Café

We’re undertaking some research with website visitors to learn more about how people find access-related information provided by museums. This survey shouldn't take longer than 10 minutes to complete. Thank you for your time!

Help us organize our website content.

Several chromatogram prints showing separation of bases of hydrolysed nucleic acids. This file contains largely unlabelled data taken from the nucleic acids prepared from herring, bull and echinoderm sperm, viruses pathogenic to insects and bacteria, wheat germ,  locusts and earthworms. Included in this file is a print of a paper chromatogram run with samples of herring sperm DNA (HNA) alongside supposed 5-methylcytosine (MC) isolated from the HNA, a deaminated form of MC (MC de-am.), synthetic 5-methylisocytosine (MisoC) and a deaminated form of MisoC (MisoC de-am). The unknown is MC, and this run provides considerable support for the hypothesis that MC is 5-methylcytosine. These results were used in Wyatt's 1951 paper "Recognition and Estimation of 5-methylcytosine in Nucleic Acids" published in <i>The Biochemical Journal </i>, showing how the substance had been characterised and estimated.

Chromatogram prints [2]

Licence and re-use