Methods

Folder compiled by Carol Churcher containing protocols for DNA sequencing with some manuscript notes. Includes Eppendorf 384 Twin-Tec plate cycling; new finishing mix protocol, 2005; inverted spin sequencing clean-up; printouts from Sanger Centre intranet with protocols from the biochemical development team; Amersham Biosciences DYEnamic ET Terminator Cycle Sequencing Kit; finishing stuff - 1/16th sequencing protocol for maps; 1/64th sequencing protocol; notes from a finishers meeting held December 1992; Sanger Centre sequencing read naming conventions; PCR protocols; DNA extraction from gel; P1, cosmid and Lambda prep; frozen TGI; M13 and pUC preps; bead prep; sequence from YACs; and using frozen electroporated cells.

c.1990-2005

1 file