Immunology Notebook: 3

Notebook filled by Alan Williams with workings, notes and comments on his Immunology work. Notes relate to affinity labelling of Immunoglobulin receptors on lymphphocytes and Mucosal immunity including: autoradiography, and immunisation of rats with Nitrohydroxyiodophenylacetate, Immunisation and skinpainting, Submaxillary protein and colostrum preparation.

Includes Williams’ first use of SDS-PAGE (polyacrylamide gel electrophoresis); and of immunoelectrophoresis, Also preparation of rabbit Fab and (Fab’)2 needed for quantitation of lymphocyte surface immunoglobulin specific binding - essential for all later experiments using monoclonal antibody purification of cell-surface molecules.

Includes first mention of anti-IgM (with Jens [Jensenius])

28 January 2071-29 October 1971

1 volume

There are 59 notebooks detailing Alan Williams immunology research including notes on Thy-1 and 4 notebooks that focus on specific subjects including a book of methodologies and books on Nucleic Acid Chemistry and Monoclonals. The Notebooks 1 to 59 including a linking system that Alan used to call back to previous notes: where research or points from a previous book were furthered or expanded upon, Williams linked to them. The new information will have the number of the notebook containing the linked material, in a circle, and then sometimes a page number. These instances have not been detailed in the catalogue description as they are sometimes too numerous to be coherant without seeing the notebook.