Live murine lymph node

  • Daniela Malide, Jean-Yves Metais, Cynthia E Dunbar, NIH, Bethesda, USA
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Live murine lymph node. Daniela Malide, Jean-Yves Metais, Cynthia E Dunbar, NIH, Bethesda, USA. Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). Source: Wellcome Collection.

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Description

3D reconstruction of confocal/multi-photon images collected through a 100 micrometer thick live lymph node from a bone-marrow transplanted mouse (4 months post transplant). Cells express one of five different fluorescent proteins which gives them their colour: Cerulean (cyan), EGFP (green), Venus (yellow), tdTomato (purple), mCherry (red). Collagen matrix surrounds the cells (white). Tile stitched image size is 680 x 720 micrometers.

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