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East African Tsetse and Trypanosomiasis Research and Reclamation Organisation

Photographs

Trypanosome handling techniques developed at EATRO

The rack for the glass capillaries used to contain the infected blood samples to be cryopreserved

Bleeding an infected mouse from the heart (E B Grainge)

Filling the heparinized, infected blood from an ampoule (kept cool by an ice-water jacket) into the capillaries held in the rack (K C Humphreyes)

Sealing the capillaries (W A F Webber)

Putting the sealed capillaries into a tube of ethanol cooled in ice/water

The tube of capillaries in ethanol inserted into the insulating jacket. The heat reservoir of the ethanol within the insulating jacket gave a suitably slow cooling rate when put into the solid CO2 cabinet

The insulating jacket in the solid CO2 cabinet

The storage box and its removable compartments used to contain each individual preservation (stabilate) in the solid CO2 cabinet; the perspex funnel used to fill the capillaries into each ethanol-containing compartment

Inserting capillaries into an ethanol-filled tube in solid CO2 for despatch to another laboratory

Retrieving a stabilate from the solid CO2 `bank' (K van Hoeve, M P Cunningham, E B Grainge)

Titration of stabilate:  weighing a capillary (the quantity of the stabilate subsequently discharged was obtained by weighing again after discharge)

Titration: discharging the contents of a capillary into medium to make the first dilution

Titration: serial dilution in tubes kept in an ice/water bath; inoculation of groups of mice at each dilution

Inspection of inoculated mouse groups (K van Hoeve)

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Inspection of inoculated mouse groups (K van Hoeve)

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