Gene transfer and expression protocols / edited by E.J. Murray.

Clifton, N.J. : Humana Press, [1991], ©1991.

xiii, 439 pages : illustrations ; 24 cm.

Copy 1. Donor: Wellcome Trust Sanger Institute.

Preparation of recombinant plasmid DNA for DNA-mediated gene transfer / Rémy Aubin, Michael Weinfeld, Malcolm C. Paterson -- Calcium phosphate mediated gene transfer into established cell lines / Hiroto Okayama, Claudia Chen -- Transfection of the Chloramphenicol-acetyltransferase gene into eukaryotic cells using diethyl-aminoethyl (DEAE)-dextran / Richard A. Lake, Michael J. Owen -- Polybrene/DMSO-assisted gene transfer / Remy Aubin, Michael Weinfeld, Malcolm C. Paterson -- Electroporation technique of DNA transfection / Sarah C. Spencer -- Irradiation and fusion gene transfer / Peter N. Goodfellow -- Direct use of [lambda] phage particles for DNA transfection / Masahiro Ishiura -- Cationic liposome-mediated transfection with Lipofectin reagent / Philip L. Felgner -- Preparation of high-molecular-weight DNA for use in DNA transfection : secondary transfections for cloning active genes by direct phenotypic selection / Michael A. Tainsky -- Chromosome-mediated gene transfer / Janet M. Shipley, Denise Sheer -- Manipulation of adenovirus vectors / Frank L. Graham, Ludvik Prevec -- Manipulation of vaccinia virus vectors / Michael Mackett -- Manipulation of baculovirus vectors / Murk J. Bailey, Robert D. Possee -- Manipulation of SV40 vectors / Marie-Louise Hammurskjöld -- Choice and manipulation of retroviral vectors / Jay P. Morgenstern, Hartmut Land -- Use of tissue-plasminogen activator as a reporter gene / Nicholas C. Wrighton -- Use of Escherichia coli (E. coli) lacZ ([beta]-galactosidase) as a reporter gene / Grant R. MacGregor, Gamy P. Nolan, Steven Fiering, Mario Roederer, Leonard A. Herzenberg -- Application of the firefly luciferase reporter gene / Vincent Giguère -- Use of vectors to confer resistance to antibiotics G418 and hygromycin in stably transfected cell lines / Robert F. Santerre, Jenna D. Walls, Brian W. Grinnell -- Selection of cells defective in pyrimidine (TK⁻) and purine (APRT⁻ and HPRT⁻ salvage : development of host strains appropriate for transfection / Mark Meuth, Janet Harwood -- Quantitative and qualitative analysis of exogenous gene expression by the S1 nuclease protection assay / Maggie E. Walmsley, Roger K. Patient -- The RNase protection assay / Mitchell H. Finer -- Primer extension analysis of mRNA isolated from transfected cell lines / Mark W. Leonard, Roger K. Patient -- Northern blot analysis of gene expression / Robb Krumlauf -- Analysis of transcriptional initiation in isolated nuclei / David Stott -- Immunoperoxidase staining of gene products in cultured cells using monoclonal antibodies / Roger Morris -- The use of flow cytometry to detect transfected gene products / Raymond Bujdoso, David Sargan, Keith Ballingall, Andrew Sanderson -- Determination of foreign gene copy number in stably transfected cell lines by Southern transfer analysis / Ronald M. Fourney, Rémy Aubin, Kevin D. Dietrich, Makolm C. Paterson -- Evaluation of extrachromosomal gene copy number of transiently transfected cell lines / Angus C. Wilson, Roger K. Patient -- Use of Dpn I restriction enzyme to assess newly replicated gene copies in amplifiable vector systems / Alison C. Brewer, Roger K. Patient -- Use of polymerase chain reaction (PCR) to detect homologous recombination in transfected cell lines / Andreas Zimmer, Peter Gruss -- Induction of erythroid-specific expression in murine erythroleukemia (MEL) cell lines / Michael Antoniou.

Includes bibliographical references and index.