Two-dimensional DNA typing : a parallel approach to genome analysis / André G. Uitterlinden and Jan Vijg.

New York ; London : E. Horwood, 1994.

xii, 197 pages : illustrations (some color) ; 24 cm.

Copy 1. Supplier/Donor: Wellcome Trust Sanger Institute.

Two-dimensional DNA typing: background and technology -- Analysis of DNA: general aspects -- Measuring DNA sequence variation -- DNA sequencing -- Restriction enzyme digestion: RFLP analysis -- Analysis of basepairing -- Evaluation -- Measuring DNA sequence variation on a total genome scale -- Two-dimensional separation of DNA fragments -- Enzymatic digestion as second dimension separation criterion -- DGGE in the second dimension -- Two-dimensional DNA typing -- Summary and discussion: general characteristics of 2D DNA typing -- Genome analysis: general considerations -- Mapping genomes -- Physical mapping -- Genetic mapping -- DNA markers -- Micro- and minisatellite sequences -- Other interspersed repetitive sequences -- Total genome analysis with DNA markers -- Combining genetic and physical mapping -- Strategies for identifying genetic traits -- Role of two-dimensional DNA typing in genome analysis -- General methodology -- Methods -- DNA isolation -- DNA digestion and sample preparation -- Electrophoretic separations -- Electrotransfer of separation patterns.

Hybridization analysis -- Experimental considerations -- Denaturing gradient gel constellation -- Buffer dependency of separation patterns -- Reproducibility -- Pattern evaluation by image analysis -- Image capturing -- Spot detection -- Calibration -- Matching -- Database generation, searching and matching -- Applications -- Genome analysis of lower organisms -- Analysis of viral and bacterial genomes -- Analysis of lower eukaryotes -- Genetic linkage analysis -- Locus-specific melting behaviour of minisatellite alleles -- Anatomy of a 2D DNA typing gel -- Association studies -- Genetic analysis of cancer -- Mutation analysis -- Future developments -- Positional 2D DNA typing: the concerted use of genetic and physical mapping -- Two-dimensional cDNA typing: analysing the expressed part of the genome -- Gene scanning: diagnosis of all possible mutations in disease genes.

Includes bibliographical references (p. [164]-194) and index.