Micro-organisms and disease : an introduction to the study of specific micro-organisms / by E. Klein.

  • Klein, E. (Edward), 1844-1925.
Date:
1896
Catalogue details

Licence: Public Domain Mark

Credit: Micro-organisms and disease : an introduction to the study of specific micro-organisms / by E. Klein. Source: Wellcome Collection.

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    place tlie excess of the dye is washed away with salt solution, water or alcohol, or both, as the case may be (see below). Unless the organisms are embedded in continuous masses of solids, this method gives good results. In the latter case, say if they are embedded in a microscopic lump of tissue, or in a particular spot of a fine section of a fresh tissue, it is necessary, after having placed the lump or section on an object-glass, to drop the dye on to this previous to putting on the cover-glass. After some minutes the dye is allow^ed to run off by inclining the object-glass, and then the w^ashing is proceeded with till all the excess of the dye is removed; the mounting is then done by placing a drop of water or salt solution on the specimen and covering it with a cover-glass. In the case of sections through fresh and hardened tissues containing micro-organisms, the method of staining and of permanently mounting them as a whole is more complicated, and will be detailed presently. When one has to deal with coherent masses of micro- organisms, present either in natural media {i.e. animal tissue) or artificial cultivations, such as zooglea and pellicles of micrococcus or bacillus, these can be bodily transferred to a watch-glass, stained, washed, and mounted without much difficulty, either for immediate or permanent use. The permanent specimens are made in this way Place the section or pellicle in a watch-glass containing the dye, leave it there till deeply tinted, take out with a needle, section lifter, or the like, wash in water, then in alcohol, leave here for sufficient time till most of the excess of the colouring-matter is removed, then lift it on to an object- glass, spread well out, place on it a drop of xylol or clove- oil, and after a minute or two drain off, add a drop of Canada-balsam solution (in chloroform or xylol), and cover