An accurate microchemical method of estimating sugar in the blood / Albert A. Epstein.

  • Epstein, Albert Arthur, 1880-1965.
Date:
1914
Catalogue details

Licence: In copyright

Credit: An accurate microchemical method of estimating sugar in the blood / Albert A. Epstein. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    Put one or two drops of the tluorid or oxalate solution into tlie graduated test-tube 4 (see illustration). By means of the blood pipet, 3, 0.2 c.c. of blood is obtained from the tip of the finger or the lobe of the ear and is discharged into the tube 4, containing the fluorid solution. The pipet is rinsed two or thi ee times with distilled water and the washings added to Apparatus for estimating sugar in the blood by microchemical method. 1. Sahli-Gower hemoglobinometer stand and graduated tube. 2. Two standard color tubes, one (A) suitable for measuring quantities of sugar in the blood ranging from 0.05 per cent, to 0.1 per cent.; the other (B) is adapted to quantities of sugar over 0.1 per cent.; 3. A special pipet (resembling but larger than the hemoglobin blood-pipet) graduated at 0.1 c.c. and 0.2 c.c for collecting and measuring the blood. 4. A test tube (J4 by 4 inches) graduated at 1.0 c.c. and 2.5 c.c. 5. Another test tube of similar dimensions (not graduated) suitable for boiling. the blood in Tube 4. Distilled water is then added to the 1.0 c.c. mark. After laking of the blood has taken place, picric acid is added to this (a few drops at a time) up to the 2.5 c.c. mark, shaking the tube gently with each addition of the acid. Precipitation of the blood-proteins takes place; the sugar, together with an excess of picric acid sufficient for the given tube is properly calibrated. In order to facilitate a direct reading of the percentage of sugar on these hemoglobinometer tubes, it is essen- tial to have 1.0 c.c. of fluid stand at mark 50. To overcome a discrep- ancy (if any exists) in the calibration of a given tube, one may put one, two or three small glass beads in the bottom of the tube, of such size as to raise the meniscus of 1.0 c.c. of fluid up to the 50 per cent. mark.