Movie of apoptosis, confocal

Movie (avi) of apoptosis, made using fluorescence microscopy. This field of cells has been exposed to strongly oxidised LDL. Cells with compromised membrane permeability (death) are dying predominantly by apoptosis. All cell nuclei are stained blue with the permeable DNA dye hoechst. Phosphatidylserine rearrangement is detected with annexin-V-fitc (green). Cells in the early stages of apoptosis flip their phosphatidylserine to the surface of the plasma membrane and stain green. Later, when the cells die the plasma membrane is compromised and the cell impermeant propidium iodide enters and stains the nuclei red. Compare the movie B0005599, which shows cell death by necrosis, but using the same stains.