A method for hemolysis and agglutination tests / A.A. Epstein and R. Ottenberg.

  • Epstein, Albert Arthur, 1880-1965.
Date:
1909
Catalogue details

Licence: In copyright

Credit: A method for hemolysis and agglutination tests / A.A. Epstein and R. Ottenberg. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    to the bottom, and pronounced hemolysis can be seen. For finer grades of hemolysis it is usually necessary to allow the tubes to stand twelve to twenty-four hours (in the ice-box).* Agglutination (which, when it occurs, is rather prompt) can be readily observed in the gross by the clumping and sedimentation of the erythrocytes. The importance that direct blood transfusion has recently assumed, and the necessity of testing for hemolysis as well as agglutination before each transfusion, should make this method one of clinical value. The ease and rapidity with which experiments can be made by this method make it of value in scientific work. The results obtained have been compared in parallel tests with those of the standard method and found to be identical. *When it is desired to make comparative tests as to the intensity of hemolysis, the total amount (as well as the proportions) in each test ought to be the same. In this case it is easy to calibrate any number of pipettes by simply transferring a small drop of fluid (normal salt solution) from the tip of one to the tip of another.