Chromosome analysis protocols / edited by John R. Gosden.

Chromosome Analysis Protocols provides clear, step-by-step instructions for all the techniques used in both classical and molecular cytogenetics. The book emphasizes the use of human material, however, most of the techniques are readily applicable to other animal chromosomes. Chapters feature methods used in the preparation of chromosomes from a number of different cell types, and techniques for producing banded chromosomes and analyzing karyotypes.

Numerous molecular techniques for analyzing chromosomes directly at the light-microscope level, and other molecular genetics methods are described in detail by scientists who regularly use them in their laboratories.

Totowa, N.J. : Humana Press, [1994], ©1994.

xi, 508 pages : illustrations (some color) ; 24 cm.

Copy 1. Donor: Wellcome Trust Sanger Institute.

Mitotic metaphase chromosome preparation from peripheral blood for high resolution / George Spowart -- Chromosome preparation from hematological malignancies / Fiona M. Ross -- Meiotic chromosome preparation / Ann C. Chandley, Robert M. Speed, Kun Ma -- Preparation of chromosomes for scanning electron microscopy / Adrian T. Sumner, Andrew R. Ross, Elizabeth Graham -- Immortalized cell lines : chromosome preparation and banding / Judy Fletcher -- Chromosome banding and identification absorption staining / Adrian T. Sumner -- Chromosome banding and identification: fluorescence / Adrian T. Sumner -- Chromosome banding: stain combinations for specific regions / Dieter Schweizer, Peter F. Ambros -- Inhibition of chromosome condensation / Adrian T. Sumner, Arthur R. Mitchell -- Analysis of chromosomes with restriction endonucleases and DNase hypersensitivity / Joaquina de la Torre, Adrian T. Sumner -- Automatic karyotype analysis / Jim Graham, Jim Piper -- Bivariate chromosome analysis using a commercial flow cytometer / Nigel P. Carter -- Chromosome sorting by flow cytometry : production of DNA libraries and gene mapping / Judith A. Fantes, Daryll K. Green, Andrew Sharkey -- Electrophoretic karyotype analysis: pulsed field gel electrophoresis / John Maule -- Immunofluorescence techniques applied to mitotic chromosome preparations / Peter Jeppesen -- Immunocytochemical techniques applied to meiotic chromosomes / Christa Heyting [and others] -- Immortalized cell lines: their creation and use in gene mapping / Veronica Van Heyningen -- Oligonucleotide primed in situ DNA synthesis (PRINS) : an alternative to in situ hybridization for gene mapping and the inversion of genome organization / John R. Gosden, Diane Lawson -- Electron microscopic localization of in situ hybrids / Sandya Narayanswami, Barbara A. Hamkalo -- Protocols for chromosome-mediated gene transfer : selection strategies, transgenome analysis, enrichment cloning and mapping / David J. Porteous -- Construction of chromosome-specific libraries of yeast artificial chromosome recombinants from somatic hybrid cell lines / Benoit Arveiler -- Yeast artificial chromosome recombinants in a global strategy for chromosome mapping: amplification of internal and terminal fragments by PCR and generation of fingerprints / Benoit Arveiler -- Chromosome dissection and cloning / Steven D.M. Brown, Alysson H. Carey -- Molecular analysis of chromosome aberrations in hematological malignancies : Southern hybridization / David L. Saltman, Stephen P. Hunger, Gillian E. Turner -- Molecular analysis of chromosome aberrations : in situ hybridization / Peter Lichter, Thomas Ried -- Alu- and L1-primed PCR-generated probes for nonisotopic in situ hybridization / John R. Gosden, Matthew Breen, Diane Lawson -- Chromosome substructure investigation : telomeres / Robin C. Allshire, Howard J. Cooke.

Includes bibliographical references and index.