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Providing experimental methods and protocols for performing pheromone research in a variety of organisms ranging from invertebrates to vertebrates, Pheromone Signaling: Methods and Protocols covers a wide spectrum of experimental approaches necessary for handling pheromone molecules, measuring receptor response and neural activation, and analyzing behavioral output. A great deal of progress has been made in understanding the molecular mechanisms underlying pheromone action, largely due to the discovery of receptor genes, the advancement of imaging techniques, and key multi-disciplinary approaches including aspects of organic chemistry, biochemistry, molecular biology, electrophysiology, and behavioral science. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Pheromone Signaling: Methods and Protocols details the key methodologies utilized in laboratories all over the world, making them accessible to those who want to begin investigation in the area of pheromone research.

Methods in Molecular Biology, Methods and Protocols

Springer eBooks

XV, 399 pages 74 illustrations, 30 illustrations in color : online resource.

Pheromone Signaling : Methods and Protocols / edited by Kazushige Touhara.

Classical methods for microbial strain engineering, used to improve the production of bioproducts, have serious drawbacks and have been found to be unsuitable for complex strain development applications. In Strain Engineering: Methods and Protocols, powerful new genetic engineering-based strain engineering methods are presented for rational modification of a variety of model organisms. These methods are particularly powerful when utilized to manipulate microbes for which sequenced and annotated genomes are available. Collectively, these methods systematically introduce genome alterations in a precise manner, allowing the creation of novel strains carrying only desired genome alterations. In the first section, E. coli-based bacterial strain engineering strategies are reviewed, while the second section presents analogous microbial engineering strategies for eukaryotic cells using the yeast Saccharomyces cerevisiae as a model. The third section covers examples of the proliferative adaptations of these base technologies to strain engineer industrially important prokaryotic or eukaryotic microbial systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Strain Engineering: Methods and Protocols serves as an ideal guide to scientists in academia, pharmaceutical science, and biotechnology who perform microbial strain engineering.

XI, 480 pages 83 illustrations, 2 illustrations in color : online resource.

Strain Engineering : Methods and Protocols / edited by James A. Williams.

Genomic imprinting, the process by which the non-equivalence of the paternal and maternal genomes is established, has been fascinating us for over three decades and has provided many emerging scientists with the chance to hit their stride in a frontier posing many unexpected questions and even more surprising answers. In Genomic Imprinting: Methods and Protocols, experts in the field provide a survey of the technologies that are being applied to advance the study of imprinting. This detailed volume features new technologies that are accelerating the pace of discovery of imprinted genes and characterization of their epigenetic profile, bioinformatic procedures for prediction and comparative analyses of imprinted genes, as well as methods in embryology and basic molecular biology that have been employed for many years, some appearing in new versions for small cell numbers. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Genomic Imprinting: Methods and Protocols will aid scientists in unveiling both much awaited answers and all-new questions to keep this vital field busy for many exciting years to come.

XI, 297 pages 40 illustrations, 19 illustrations in color : online resource.

Genomic Imprinting : Methods and Protocols / edited by Nora Engel.

The discovery of stem and progenitor cells in the adult mammalian CNS challenged the long standing no new neuron doctrine and opened the door to the potential for cell replacement therapy. The process from discovery to clinical applications can be long and tortuous, requiring rigorous steps involving standardized and precise protocols. Neural Progenitor Cells: Methods and Protocols is a collection of practical articles describing techniques used to study neural stem and progenitor cells. The volume also highlights the promise of stem cell-based therapeutic applications for CNS disorders. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Neural Progenitor Cells: Methods and Protocols serves both professionals and novices with its well-honed methodologies.

XI, 241 pages 49 illustrations, 36 illustrations in color : online resource.

Neural Progenitor Cells : Methods and Protocols / edited by Brent A. Reynolds, Loic P. Deleyrolle.

Today, progress in rAAV-mediated gene transfer is so robust that long-term, efficient, and regulatable transgene expression is reproducibly achieved in large animal models. The complexity of gene transfer agents in the context of their clinical use requires investigators from a wide variety of backgrounds to have an understanding — or at least an appreciation of — the regulatory environment and constraints that affect vector design, manufacturing, pre-clinical testing, and clinical use, with an emphasis on patient protection. In Adeno-Associated Virus: Methods and Protocols, experts from the United States and Europe have contributed current knowledge of this multi-dimensional field relating to the biology of AAV, rAAV vector design, vector manufacturing and product testing, performance of rAAV vectors in major organs, rAAV-related immunological issues, design of animal and clinical studies, and clinical experience. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Adeno-Associated Virus: Methods and Protocols provides a complete and comprehensive understanding of this multi-disciplinary and rapidly progressing field.

XIII, 462 pages 43 illustrations, 20 illustrations in color : online resource.

Adeno-Associated Virus : Methods and Protocols / edited by Richard O. Snyder, Philippe Moullier.

Although initially described as major regulators of cytoskeletal remodeling, Rho GTPases have been implicated in the establishment of polarity, endocytosis, vesicle trafficking, morphogenesis, cytokinesis, transcriptional activation, cell cycle progression, and apoptosis, to mention a few. In addition, Rho GTPases have acquired medical relevance because of their participation in tumorigenesis and metastasis, in cardiovascular conditions, and as targets of infectious agents. The field has broadened even more with the contribution of studies in model organisms (plants, amoebas, fungi, invertebrates), each adding a particular view to the complexity of the family and vastly enriching our perception of these important signaling components. Divided into five convenient sections, Rho GTPase: Methods and Protocols provides an historical overview of the field and an account of the phylogenetics of the Rho family, general biochemical methods, and functional assays that allow monitoring the consequences of manipulating Rho GTPases in a variety of contexts. Additionally, the volume devotes a section to advanced imaging methods and to recently developed high throughput methods, closing with techniques specifically designed for studies in selected non-mammalian model organisms. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Rho GTPase: Methods and Protocols provides techniques that are standard for researchers in the field but also includes protocols for those who, being already familiar with some of the techniques, wish to explore additional aspects.

XIV, 407 pages 78 illustrations, 2 illustrations in color : online resource.

Rho GTPases : Methods and Protocols / edited by Francisco Rivero.

The liver is responsible for a wide range of critical functions essential to life, and is composed of several different cell types. In Liver Proteomics: Methods and Protocols, expert researchers in the field detail many of the methods that are used to study the live. These methods include the most up-to-date strategies being used to characterize the liver proteome at the global, cellular, subcellular, post translational and functional level.Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Liver Proteomics: Methods and Protocols seeks to aid scientists in the further study of this crucially important organ.

XIII, 346 pages 51 illustrations, 22 illustrations in color : online resource.

Liver Proteomics : Methods and Protocols / edited by Djuro Josic, Douglas C. Hixson.

At the intersection of metabolite analysis, metabolic fingerprinting, and metabolomics, the study of metabolic profiling has evolved steadily over the course of time as have the methods and technologies involved in its study. In Metabolic Profiling: Methods and Protocols, expert researchers in the field present protocols that are illustrative of the evolution of metabolic profiling from single molecule analysis to global metabolome profiling. Comprised of the most essential techniques, this volume covers topics from inborn errors of metabolism and drug metabolite analysis to nuclear magnetic resonance metabolic profiles. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Metabolic Profiling: Methods and Protocols serves as a resource for both established and new investigators in this vital and ever-developing field.

Metabolic Profiling : Methods and Protocols / edited by Thomas O. Metz.

Cell culture methodologies have become standard procedures in most plant laboratories. Currently, facilities for in vitro cell cultures are found in practically every plant biology laboratory, serving different purposes since tissue culture has turned into a basic asset for modern biotechnology, from the fundamental biochemical aspects to the massive propagation of selected individuals. Plant Cell Culture Protocols, Third Edition is divided into five convenient sections that cover topics from general methodologies, such as culture induction, growth and viability evaluation, statistical analysis and contamination control, to highly specialized techniques, such as clonal propagation, haploid production, somatic embryogenesis, organelle transformation. The volume concludes with a section on the laborious process of measuring the epigenetics changes in tissue cultures.Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Cell Culture Protocols, Third Edition seeks to serve both professionals and novices with its guide to the most common and applicable techniques and methods for plant tissue and cell culture.

XIV, 430 pages 46 illustrations, 18 illustrations in color : online resource.

Plant Cell Culture Protocols / edited by Víctor M. Loyola-Vargas, Neftalí Ochoa-Alejo.

In Virus Hybrids as Nanomaterials: Methods and Protocols expert researchers in the field detail many of the methods used to study virus for medial and nonmedical applications. These include methods and techniques for genetically engineering viruses for therapeutic purpose and vaccine production, chemically modified viruses for virus-templated nanoparticles production, and genetically engineered or chemically modified viral particles as imaging agents. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Virus Hybrids as Nanomaterials: Methods and Protocols seeks to aid new researchers to get involved in this multidisciplinary area.

X, 235 pages 44 illustrations, 21 illustrations in color : online resource.

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Pheromone Signaling : Methods and Protocols / edited by Kazushige Touhara.

Strain Engineering : Methods and Protocols / edited by James A. Williams.

Genomic Imprinting : Methods and Protocols / edited by Nora Engel.

Neural Progenitor Cells : Methods and Protocols / edited by Brent A. Reynolds, Loic P. Deleyrolle.

Adeno-Associated Virus : Methods and Protocols / edited by Richard O. Snyder, Philippe Moullier.

Rho GTPases : Methods and Protocols / edited by Francisco Rivero.

Liver Proteomics : Methods and Protocols / edited by Djuro Josic, Douglas C. Hixson.

Metabolic Profiling : Methods and Protocols / edited by Thomas O. Metz.

Plant Cell Culture Protocols / edited by Víctor M. Loyola-Vargas, Neftalí Ochoa-Alejo.

Virus Hybrids as Nanomaterials : Methods and Protocols / edited by Baochuan Lin, Banahalli Ratna.

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Pheromone Signaling : Methods and Protocols / edited by Kazushige Touhara.

Strain Engineering : Methods and Protocols / edited by James A. Williams.

Genomic Imprinting : Methods and Protocols / edited by Nora Engel.

Neural Progenitor Cells : Methods and Protocols / edited by Brent A. Reynolds, Loic P. Deleyrolle.

Adeno-Associated Virus : Methods and Protocols / edited by Richard O. Snyder, Philippe Moullier.

Rho GTPases : Methods and Protocols / edited by Francisco Rivero.

Liver Proteomics : Methods and Protocols / edited by Djuro Josic, Douglas C. Hixson.

Metabolic Profiling : Methods and Protocols / edited by Thomas O. Metz.

Plant Cell Culture Protocols / edited by Víctor M. Loyola-Vargas, Neftalí Ochoa-Alejo.

Virus Hybrids as Nanomaterials : Methods and Protocols / edited by Baochuan Lin, Banahalli Ratna.

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