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Powerful new techniques in molecular biology have revolutionized our ability to investigate and define cellular processes at the molecular level for medical purposes. In Medical Biomethods Handbook, John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are Southern and Western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy. State-of-the-art and highly practical, Medical Biomethods Handbook offers novice and experienced researchers an in-depth understanding of the theory, practice, and application of the key molecular biology techniques used in biomedical research and diagnostic investigation today.

Springer eBooks

Medical Biomethods Handbook / edited by John M. Walker, Ralph Rapley.

Understanding the relationship between a microorganism and its environment is essential to the successful manipulation of industrial, biochemical, and medical processes. In Environmental Microbiology: Methods and Protocols, highly practiced experimentalists who often have perfected the methods they write about describe readily reproducible techniques for determining most of the important factors governing microorganisms and their habitats. Presented in step-by-step detail, these cutting-edge methods range from those for the study of marine organisms, to those for investigating microorganisms occurring in groundwater, to the biodiversity found in remote environments. The protocols for studying fermented milks are significant for investigators concerned with milk as an item of food for infants, small children, and even adults. Additional methods for the recovery and determination of nucleic acids and other compounds affecting, and affected by, microorganisms, are provided for certain enzymes produced by plant pathogens and for obtaining microbial species tolerant of such inhibitors as heavy metals. Review articles discuss the endophytic bacterium Bacillus mojavensis, the engineering of bacteria to enhance their ability to carry out bioremediation of aromatic compounds, and the use of chemical shift reagents and Na-NMR to study sodium gradients in microorganisms. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Environmental Microbiology: Methods and Protocols offers microbiological researchers a powerful set of techniques for investigating and understanding microorganisms in their native environments.

Methods in biotechnology

Environmental Microbiology : Methods and Protocols / edited by John M. Walker, John F. T. Spencer, Alicia L. Ragout de Spencer.

Medical Heritage Library

56 pages, 1 unnumbered folded leaf of plate : illustrations ; 20 cm (8vo)

An experimental inquiry into the similarity in virtue between the Cornus florida and sericea : and the Cinchona officinalis of Linnaeus, together with an inquiry into the modus operandi of astringent vegetables in preventing the putrefactive fermentation / by John M. Walker.

Neisseria meningitidis is a major cause of childhood morbidity and mortality in industrialized nations and is responsible for epidemics of meningococcal meningitis and septic shock in Africa and Asia. In Meningococcal Disease: Methods and Protocols, Andrew Pollard and Martin Maiden bring together a panel of leading authorities to produce a comprehensive, interdisciplinary survey of recent advances and knowledge about this much-feared disease. The review and methods-based chapters collected here provide essential information for diagnosis in the clinical microbiology laboratory, isolate characterization, clinical management, and control of meningococcal disease. They also examine the immunopathological mechanisms occurring in the acutely ill, host-pathogen interactions, and the possible components of meningococcus responsible for virulence. A companion volume, Meningococcal Vaccines, provides detailed methods for the design and evaluation of meningococcal vaccines. Comprehensive and authoritative, Meningococcal Disease: Methods and Protocols integrates the basic science and the clinical and epidemiological aspects of this terrible infectious disease to promote the deeper understanding needed to identify novel targets for therapeutic interventions and vaccines.

Methods in Molecular Medicine™

Meningococcal Disease : Methods and Protocols / edited by John M. Walker, Andrew J. Pollard, Martin C. J. Maiden.

Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. Molecular Biomethods Handbook, 2nd Edition expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Part A of the book describes nucleic acid methods, such as gene expression profiling, microarray analysis and quantitative PCR. In Part B, protein and cell-based methods are outlined, in subjects ranging from protein engineering to high throughput screening. Written by a well-established panel of research scientists, Molecular Biomethods Handbook, 2nd Edition provides an up-to-date collection of methods used regularly in the authors’ own research programs. This book will prove to be an invaluable reference for those engaged in or entering the field of molecular biology, and will provide the necessary background for those interested in setting up and using the latest molecular techniques.

Molecular Biomethods Handbook / edited by John M. Walker, Ralph Rapley.

The molecular biology revolution has transformed developmental biology into one of the most exciting and fruitful fields in experimental biomedical research today. In Developmental Biology Protocols, established leaders in this field demonstrate this achievement with a comprehensive collection of cutting-edge protocols for studying and analyzing the events of embryonic development. Drawing on state-of-the-art cellular and molecular techniques, as well as new and sophisticated imaging and information technologies, this first volume of three presents readily reproducible methods for establishing and characterizing several widely used experimental model systems, for both the study of developmental patterns and morphogenesis, and the examination of embryo structure and function. In addition, there are step-by-step methods for the analysis of cell lineage, the production and use of chimeras, and the experimental molecular manipulation of embryos, including the application of viral vectors. The second and third volumes in this seminal set complete today's widest-ranging collection of techniques designed to decipher the exact cellular, molecular, and genetic mechanisms that control the form, structure, and function of the developing embryo. Volume 2 describes state-of-the-art methods for the study of organogenesis, the analysis of abnormal development and teratology, the screening and mapping of novel genes and mutations, and the application of transgenesis, including the production of transgenic animals and gene knockouts. No less innovative, Volume 3 introduces powerful techniques for the manipulation of developmental gene expression and function, the analysis of gene expression, the characterization of tissue morphogenesis and development, the in vitro study of differentiation and development, and the genetic analysis of developmental models of diseases. Highly practical and richly annotated, the three volumes of Developmental Biology Protocols describe multiple experimental systems and details techniques adopted from the broadest array of biomedical disciplines. Every researcher will not only better understand the principles, background, and rationale for how form and function are elaborated in an organism, but also gain full practical access to today's best methods for its analysis.

Methods in Molecular Biology™

Developmental Biology Protocols : Volume I / edited by John M. Walker, Rocky S. Tuan, Cecilia W. Lo.

In is now understood that the response of mammalian cells to a wide variety of potentially toxic agents may be intimately linked with many human diseases, including rheumatoid arthritis, ischemia, fever, infection, and cancer. In Stress Response: Methods and Protocols, Stephen Keyse has assembled a diverse collection of readily reproducible methods devoted to the study of these varied and powerful responses. Written by leading researchers expert in the techniques they describe, these detailed methods cover the detection and assay of stress-induced damage, the activation of a wide range of signal transduction pathways by cellular stress, stress-induced gene expression, and stress protein function. To ensure experimental success, step-by-step guidance is provided for each method, along with details of reagents, equipment, and other requirements. The methods include both well-established techniques and new technologies at the leading edge of research. Wide ranging and highly practical, Stress Response: Methods and Protocols provides a gold-standard bench manual for today's basic and clinical scientists working to understand how cells and tissues respond during physiological stress and in human disease

Stress Response : Methods and Protocols / edited by John M. Walker, Stephen M. Keyse.

Plant Cell and Tissue Culture / edited by Jerey W. Pollard, John M. Walker.

Methods in molecular biology

Animal Cell Culture / edited by John M. Walker, Jeffrey W. Pollard, John M. Walker.

In the Molecular Biomethods Handbook, Ralph Rapley and John Walker assemble an authoritative team of investigators to illuminate the core bioanalytical techniques used every day in their own laboratories, and laboratories throughout the world. These highly experienced writers fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few. Numerous key protein methods, as well as support and related techniques, are also included. The Molecular Biomethods Handbook provides both established scientists and novices who are new to these techniques a deeper understanding of the widest variety of biotechniques-and how to use them successfully. The resulting insights will significantly enhance your ability to investigate and define biological processes at the molecular level.

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Medical Biomethods Handbook / edited by John M. Walker, Ralph Rapley.

Environmental Microbiology : Methods and Protocols / edited by John M. Walker, John F. T. Spencer, Alicia L. Ragout de Spencer.

An experimental inquiry into the similarity in virtue between the Cornus florida and sericea : and the Cinchona officinalis of Linnaeus, together with an inquiry into the modus operandi of astringent vegetables in preventing the putrefactive fermentation / by John M. Walker.

Meningococcal Disease : Methods and Protocols / edited by John M. Walker, Andrew J. Pollard, Martin C. J. Maiden.

Molecular Biomethods Handbook / edited by John M. Walker, Ralph Rapley.

Developmental Biology Protocols : Volume I / edited by John M. Walker, Rocky S. Tuan, Cecilia W. Lo.

Stress Response : Methods and Protocols / edited by John M. Walker, Stephen M. Keyse.

Plant Cell and Tissue Culture / edited by Jerey W. Pollard, John M. Walker.

Animal Cell Culture / edited by John M. Walker, Jeffrey W. Pollard, John M. Walker.

Molecular Biomethods Handbook / edited by Ralph Rapley, John M. Walker.

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Medical Biomethods Handbook / edited by John M. Walker, Ralph Rapley.

Environmental Microbiology : Methods and Protocols / edited by John M. Walker, John F. T. Spencer, Alicia L. Ragout de Spencer.

An experimental inquiry into the similarity in virtue between the Cornus florida and sericea : and the Cinchona officinalis of Linnaeus, together with an inquiry into the modus operandi of astringent vegetables in preventing the putrefactive fermentation / by John M. Walker.

Meningococcal Disease : Methods and Protocols / edited by John M. Walker, Andrew J. Pollard, Martin C. J. Maiden.

Molecular Biomethods Handbook / edited by John M. Walker, Ralph Rapley.

Developmental Biology Protocols : Volume I / edited by John M. Walker, Rocky S. Tuan, Cecilia W. Lo.

Stress Response : Methods and Protocols / edited by John M. Walker, Stephen M. Keyse.

Plant Cell and Tissue Culture / edited by Jerey W. Pollard, John M. Walker.

Animal Cell Culture / edited by John M. Walker, Jeffrey W. Pollard, John M. Walker.

Molecular Biomethods Handbook / edited by Ralph Rapley, John M. Walker.

Stay in the know

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