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Quail fibroblast responses to suberyldicholine
(SDC), shown by Ca++i signal, shown by
fluorescence. A cells at rest. B peak response to
dose of 10 microM SDC. C incubation in Na++ free
media no effect on resting Ca level, D but blocked
SDC response. E Ca++ same after washout of SDC and
Na+.
Credit: Biosciences Imaging Group,Soton Univ/WTMPL

Fibroblast responses to acetylcholine, Na+

Quail fibroblast responses to suberyldicholine
(SDC). A Phase contrast morphology. B Control
Ca++i signal. C Peak Ca signal. D Washing cells
returns Ca level to control value (purple (100nM)
to pink (over 1 microM). E cells after ionomycin
to saturate dye. F After EGTA calibration.
Credit: Biosciences Imaging Group,Southampton
University/Wellcome Trust Medical Photo Library

Fibroblast responses to acetylcholine

QUail fibroblast cells, inhibition of Ca++
emission in response to suberyldicholine (SDC) by
d-tubocurarine (d-TC). A control Ca++ signal. B
response to 10 micromolar SDC. C after incubation
with d-TC. D-H increasing concentrations of d-TC
(1nM, 10nM, 100nM, 1 micromolar, 10 micromolar).
Please credit: Biosciences Imaging Group,
Southampton University/WTMPL

Inhibition of Ca emission by fibroblasts

no caption

Credit: Biosciences Imaging Group, Southampton
University/Wellcome Trust Medical Photo Library

Cells

Dendrites of interneurons in the hippocampus.

Dendrites of interneurons in hippocampus

Time course of the Ca++i signal in Indo-1 loaded
quail fibroblast cells using the line scan
facility. Left - Confocal image showing whole
field of cells, indicating example of line
measured. Right, line scan for single cell through
time, 512 lines at 145 msec intervals.
Suberyldicholine added at line 25 (arrow).
Credit: Biosciences Imaging Group,Soton Univ/WTMPL

Ca++ fluroescence response to Indo-1,fibrobl

SEM (scanning electron micrograph) of amoeba

Please credit: Biosciences Imaging Group,
Southampton University/Wellcome Trust Medical
Photographic Library

Amoeba SEM

Cells transfected with GFP, visualised using FITC
optics. Transfected cells show clear fluorescence
which fills the cytoplasm of the cells.

Please credit: Biosciences Imaging Group,
Southampton University/WTMPL

GFP fluorescence studies, transfected cells

SEM (scanning electron micrograph) of freshwater
diatoms, with false colours.


Please credit: Biosciences Imaging Group,
Southampton University/Wellcome Trust Medical
Photographic Library

Freshwater diatoms SEM

Please credit: Biosciences Imaging Group,
Southampton University/Wellcome Trust Medical
Photographic Library

F-actin labelled fibroblast.

Quail fibroblast responses to suberyldicholine
(SDC), shown by Ca++i signal, shown by
fluorescence. FITC-alpha-bgt selectively labels
QF18 but not QT6 cells. Top row shows pairs of
GF18 cells, phase contrast & 520nm fluorescence
signal, bottom row QT6 cells, not labelled.
Credit: Biosciences Imaging Group, Southampton
University/Wellcome Trust Medical Photo Library

Section through hippocampus, showing neurons.

Neurons in hippocampus

Fibroblast cells stained with propidium iodide,
which only crosses membrane of dead and dying
cells in cluture. Most cells are unstained. Those
that do not respond to suberydicholine (SDC) are
viable.

Credit: Biosciences Imaging Group,Southampton
University/Wellcome Trust Medical Photo Library

Contrast/fluorescence cell viability

Interneurons.

Quail fibroblast responses to suberyldicholine
(SDC), shown by C++i signal, shown by
fluorescence. A Reponse of untreated cells to 10
microM SDC.

Credit: Biosciences Imaging Group, Southampton
University/Wellcome Trust Medical Photo Library

A stereo image of an interneuron in the brain, taken using a confocal microscope.

Interneuron, stereo confocal image

Quail fibroblast cells Ca++ fluorescence - showing
how nifedipine blocks the suberyldicholine (SDC)
response. A control Ca++ signal. B Response to 10
micromolar SDC, C-G increasing concentration of
nifedipine (100pM, 10nM, 1 microM, 10 microM, 100
microM).
Credit: Biosciences Imaging Group,Southampton
University/Wellcome Trust Medical Photo Library

Response of ACh receptors to nifedipine

Quail fibroblast cells - pancuronium bocking
response (Ca++i fluorescence) to 10 microM
suberyldicholine (SDC). A control Ca signal. B
response to 10 microM SDC. C addition of
pancuronium alone had no effect. D-G increasing
concs pancuronium block response more and more.
Credit: Biosciences Imaging Group,Southampton
University/Wellcome Trust Medical Photo Library

Pancuronium inhibition of ACh receptorsn

Pollen grains on buttercup anther, SEM (scanning
electron micrograph), black-and-white image.

Please credit: Biosciences Imaging Group,
Southampton University/Wellcome Trust Medical
Photographic Library

Pollen grain on buttercup anther, SEM

Quail fibroblast responses to suberyldicholine
(SDC). The increase in Ca++i signal seen in cells
in response to 10 microM SDC is completely
abolished when cells are pretreated with 200nM
alpha-bgt. Frames are at 5 second intervals, and
10 microM SDC is added between frames A and B.
Credit: Biosciences Imaging Group,Southampton
University/Wellcome Trust Medical Photo Library

Quail fibroblast responses to suberyldicholine
(SDC). A Phase contrast morphology. B Control
Ca++i signal. C Peak Ca signal. D Washing cells
returns Ca level to control value (purple (100nM)
to pink (over 1 microM). E cells after ionomycin
to saturate dye. F After EGTA calibration.(B/W)
Credit: Biosciences Imaging Group,Southampton
University/Wellcome Trust Medical Photo Library

An interneuron in the hippocampus, computer-coloured.

Interneuron in hippocampus

SEM (scanning electron micrograph) of freshwater
diatoms, with false colours. A 3D (stereoscopic
image, for viewing with red/green glasses.

Please credit: Biosciences Imaging Group,
Southampton University/Wellcome Trust Medical
Photographic Library

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Fibroblast responses to acetylcholine, Na+

Fibroblast responses to acetylcholine

Inhibition of Ca emission by fibroblasts

Cells

Dendrites of interneurons in hippocampus

Ca++ fluroescence response to Indo-1,fibrobl

Amoeba SEM

Cells

GFP fluorescence studies, transfected cells

Cells

Freshwater diatoms SEM

F-actin labelled fibroblast.

Fibroblast responses to acetylcholine

Neurons in hippocampus

Contrast/fluorescence cell viability

Interneurons.

Fibroblast responses to acetylcholine

Interneuron, stereo confocal image

Response of ACh receptors to nifedipine

Pancuronium inhibition of ACh receptorsn

Pollen grain on buttercup anther, SEM

Fibroblast responses to acetylcholine

Fibroblast responses to acetylcholine

Interneuron in hippocampus

Freshwater diatoms SEM 3D image

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More filters

Active filters:

Fibroblast responses to acetylcholine, Na+

Fibroblast responses to acetylcholine

Inhibition of Ca emission by fibroblasts

Cells

Dendrites of interneurons in hippocampus

Ca++ fluroescence response to Indo-1,fibrobl

Amoeba SEM

Cells

GFP fluorescence studies, transfected cells

Cells

Freshwater diatoms SEM

F-actin labelled fibroblast.

Fibroblast responses to acetylcholine

Neurons in hippocampus

Contrast/fluorescence cell viability

Interneurons.

Fibroblast responses to acetylcholine

Interneuron, stereo confocal image

Response of ACh receptors to nifedipine

Pancuronium inhibition of ACh receptorsn

Pollen grain on buttercup anther, SEM

Fibroblast responses to acetylcholine

Fibroblast responses to acetylcholine

Interneuron in hippocampus

Freshwater diatoms SEM 3D image

Stay in the know

Wellcome collectionWellcome Collection

Finding us:

Opening times:

Today's opening times

Wellcome collection