PCR mutation detection protocols / edited by Bimal D. M. Theophilus and Ralph Rapley.

Totowa, N.J. : Humana Press, [2002], ©2002.

x, 212 pages : illustrations ; 24 cm.

Copy 1 Donor: Wellcome Trust Sanger Institute.

Agarose and polyacrylamide gel electrophoresis / Andrea M. Guilliatt -- Internal labeling of DNA probes / Ralph Rapley -- End-labeling of DNA probes / Adrian J. Harwood -- Southern blotting of agarose gels bu capillary transfer / Ralph Rapley -- Restriction fragment length polymorphism / Mohammad S. Enayat -- PCR: principles, procedures, and parameters / Nicola Louise Jones -- Allele-specific oligonucleotide PCR / Elaine K. Green -- Long-range PCR / Peter A. Davies -- Analysis of nucleotide sequence variations by solid-phase minisequencing / Anu Wartiovaara -- Cycle sequencing of PCR products / G.K. Surdhar -- Fluorescent in situ hybridization / Sara A. Dyer -- The protein truncation test / Carol A. Hardy -- Mutation detection in factor vii cDNA from lymphocytes of hemophilia A patients by solid phase fluorescent chemical cleavage of mismatch / Naushin H. Waseem -- Denaturing gradient gel electrophoresis / Yvonee Wallis -- Conformation-sensitive gel electrophoresis / Ian J. Williams -- SSCp/heteroduplex analysis -- Cleavase fragment length polymorphism analysis for genotyping and mutation detection / Laura Heisler -- Automated genotyping using the DNA MassArray technology / Christian Jurinke -- An introduction to bioinformatics / Henry Brzeski.

Includes bibliographical references and index.