The principles of bacteriology : a practical manual for students and physicians / by A.C. Abbott.

Date:
1892
Catalogue details

Licence: Public Domain Mark

Credit: The principles of bacteriology : a practical manual for students and physicians / by A.C. Abbott. Source: Wellcome Collection.

Provider: This material has been provided by the Royal College of Physicians of Edinburgh. The original may be consulted at the Royal College of Physicians of Edinburgh.

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    distributioa of the orgaDisms and the better the results. The oese is then again steriHzed, and three of its loop fills are passed, without touching the sides of the tube, from the original into tube No. % where they are carefully mixed. Agaiu the oese is sterilized and again three dips are made from tube 2 into tube 3. This completes the dilution. The oese is now steril- ized before laying it aside. Fig. 11. During this manipulation, which must be done quickly if agar-agar is employed, the temperature of the water in the bath in which the tubes stand should never get lower than 39° C, and never higher than 43° C. If it falls too low, below 38° C, the agar gelatinizes, and can only be rcdissolvcd by a temperature which would be destructive to the organisms which may have been in- troduced into the tubes. This is not of so much moment with gelatin, as it may readily be redissolved