Volume 1

Molecular genetics / edited by J. Herbert Taylor.

  • Taylor, J. Herbert (James Herbert), 1916-
Date:
1963-1979
Catalogue details

Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Credit: Molecular genetics / edited by J. Herbert Taylor. Source: Wellcome Collection.

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    The Replication of DNA in Cell-Free i MAURICE J. BESSMAN McCollum-Pratt Institute, The Johns Hopkins University, Baltimore, Maryland I. Introduction 1 II. Synthesis of Deoxyribonucleotides 3 A. Origin of the Deoxyribosides 3 B. Synthesis of Thymidylic Acid 6 C. Synthesis of 5-Hydroxymethyldeoxycytidylic Acid (dHMP) . . 10 III. Phosphorylation of Deoxyribonucleotides 12 A. Deoxynucleoside Monophosphate Kinases 12 B. Deoxynucleoside Diphosphate Kinases 16 IV. Synthesis of DNA by Escherichia coli Polymerase 17 A. Measurement of DNA Synthesis 17 B. Requirements of the Reaction 20 C. The Limited Reaction 23 D. Nature of the Primer 25 E. Nature of the Product 27 F. Primer-Product Relationships 29 G. Synthesis of Deoxyadenylate-Deoxythymidvlate and Deoxyguanyl- ate-Deoxycytidylate Polymers 40 H. Substitution of Analogs 46 V. Synthesis of DNA by Calf Thymus Polymerase 50 VI. Synthesis of DNA in Bacteriophage-Infected Escherichia coli ... 54 A. Bacteriophage-Specific Polymerase 54 B. Glucosylation of 5-Hydroxymethylcytosine (HMC) 56 VII. Conclusions 60 Acknowledgments 60 References 60 I. Introduction 2 Most organisms can synthesize all the constituents of the DNA macro molecule from small molecular weight precursors which are also the raw 1 Contribution No. 371 of the McCollum-Pratt Institute, The Johns Hopkins University, Baltimore 18, Maryland. 2 The following abbreviations have been used in this chapter: RNA, DNA: ribonucleic acid, deoxyribonucleic acid; RNase, DNase: ribonuclease, deoxyribo- 1