Volume 1

Handbook for the physiological laboratory / by E.E. Klein, J. Burdon-Sanderson, M. Foster, and T.L. Brunton ; edited by J. Burdon-Sanderson.

  • Klein, E. (Edward) 1844-1925.
Date:
1873
Catalogue details

Licence: Public Domain Mark

Credit: Handbook for the physiological laboratory / by E.E. Klein, J. Burdon-Sanderson, M. Foster, and T.L. Brunton ; edited by J. Burdon-Sanderson. Source: Wellcome Collection.

Provider: This material has been provided by Royal College of Physicians, London. The original may be consulted at Royal College of Physicians, London.

    103/628 (page 87)
    PART II. PEEPAEATION OF THE COMPOUND TISSUES. CHAPTER VI. METHODS. The methods of examining tissues in the fresh state, with or without the addition of reagents, and of isolating the elements by the process of teasing with needles, have been fully described in the First Part. We have also seen that, in transparent structures, particularly membranes, the anatomical relations of the elements may be studied, either by observing them in the natural condition, or after preparation with the solution of chloride of gold, or with that of nitrate of silver. For the investigation of the compact tissues, other modes of preparation are necessary, in order to bring them into such a condition that fine sections can be made of them. It is the purpose of this chapter to describe the method by which this is accomplished. Preparation of Sections of Fresh Tissues.—There are a few organs or parts of organs which possess such a consistence, that it is possible, without preparation, to make microscopical sec- tions of them; such as cartilage, some tumours, skin, hyper- trophied lymphatic glands, prostate gland, kidney, liver, and under certain circumstances involuntary muscle. Sections of these tissues serve either for the study of the condition of the elements, or the action of reagents; or are made with a view to treating them with gold or silvei'. They are, however, mainly useful as facilitating the preparation of the individual elements by the process of teasing. For this purpose the section may be either used in the fresh state with indifferent liquids, or after maceration in iodized scrum, Miiller's fluid, or one per cent, solution of bichromate of potash. For the study of the anatomical relation of fresh tissues, other