Volume 5

Second interim report of the Royal Commission appointed to inquire into the relations of human and animal tuberculosis. Part II. Appendix.

  • Great Britain. Royal Commission on Tuberculosis (Human and Animal)
Date:
1904-1913
Catalogue details

Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Credit: Second interim report of the Royal Commission appointed to inquire into the relations of human and animal tuberculosis. Part II. Appendix. Source: Wellcome Collection.

Provider: This material has been provided by London School of Hygiene & Tropical Medicine Library & Archives Service. The original may be consulted at London School of Hygiene & Tropical Medicine Library & Archives Service.

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    ViKUs B. VII. Mesenteric Gland. Strain. Generation. Serum. Glycerin Serum. Glycerin Broth. I Glycerin Agar. Glycerinated Potato. Direct 1 j 12th Uniform grey layer. Uniform layer in 3 weeks identical in character and amount with that on serum. In 3 Aveeks the surface was cov- ered with a very thin grey pellicle which showed no further change. The growth on 2 per cent, glycerin gelatine was iden- tical in ai^pearance excepting that the pellicle was dotted with opaque white foci. At the end of the 1st week there was a slight initial grey growth,but no further progress until the 3rd week. At the end of the 4th week there was a grey layer not quite uniform. In 5 weeks it was rather thicker and grejash white in parts, due to small confluent colonies. At the end of 2 months delicate grey colonies had appeared in the thinner patches. Minute grey colonies were visible in the 3rd week. There had been no change 11 weeks later. Cultures were also isolated from G. P.'s 1016 and 1017, both inoculated with original material. The cultures were identical with Virus VII. direct, but a rather better growth was obtained with G.P. 1017 on potato. Virus B. VIII. Mesenteric Gland. Strain. Generation. Serum. Glycerin Serum. Glycerin Broth. Glycerin Agar. Glycerinated Potato. Direct 15th A moder- ately thick layer in 3 weeks. There was a uni- form growth a little less than on serum in the 1st week. In the 3rd week the growth became whiter and raised in parts, but on the whole less than on serum. In 6 weeks numerous small raised colonies appeared on the surface. Inoculated from a serum tube on to a tiaskof 2 per cent, glycerin broth. In 14 days there was thin grey translucent pellicle speckled with whitish foci, cover- ing about ^ of the surface. In 4weeks the surface was covered with a greyish translucent membrane studded with raised opaque white colonies. In 7 days there was a thin uniform grey ground-glass layer, which in- creased in thick- ness slightly until the 3rd week. In the 4th, 5th and 6th weeks there was no change. At the end of 10 weeks the surface was studded with minute whitish colonies. In 14 days tliere was a greyish layer composed of minute colonies. In the 4th week it was a little thicker and greyish-white, with no subseijuent increase. A culture isolated from Dog 102, fed with culture of original material, showed no alteration. Virus B. IX. Mediastinal Gland. Strain. Generation. Serum. Glycerin Serum. Glycerin Broth. Glycerin Agar. Glycerinated Potato. Direct. 14th. Thin uni form grey layer not increasing after the 2nd week. At the end of 7 days there was a grey layer witli white points de- cidedly better than on serum alone. During the 2nd and 3rd weeks the growth became thicker and the colonies whiter and more raised. At the end of the 4th week there was a greyish-white moist layer, at least twice as thick as on serum alone. On an asparagin medium the sur- face was covered with a thin grey membrane with a few whitish points. On 5 per cent, glycerin broth in 14 days the surface was iiartly covered with fairly thick opatpie white is- lands, which sank a few days later. , T here was a slight increase at the end of the 1st week. In the 2nd week there was a uniform grey ground-glass layer. In crease took place until the end of the 6th week, when there was amoder- ately thick grey granular layer, thicker and whiter in places. At the end <jf the 4th week there was a thin greyish white layer on the surface of the potato. This in- creased very little subsequently. Strains of this virus have been isolated from 15 difi'ereirt animals, including the chimpanzee, monkey, baboon, dog, cat, pig, goat, mongoose, hedgehog, rabbit and guinea-pig; with-one exception, that of Dog 50, these have reproduced the characters of the original virus, the pellicle formed on the surface of broth, though varying in thickness, exhibiting the same marked tendency to sink.