PCR analysis to sex embryos

  • Alan Handyside
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PCR analysis to sex embryos. Alan Handyside. CC0 1.0 Universal. Source: Wellcome Collection.

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Gel electrophoresis of PCR products. The upper band indicates the presence of a Y-chromosome specific repetitive sequence showing that the DNA comes from a male embryo of embryos. DNA was isolated from single cells from cleavage stage human embryos that had been generated by IVF. In this case male offspring will carry an X-linked disease. The determination of sex therefore allows selective implantaion of female embryos.

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