The malarial fevers of Baltimore : An analysis of 616 cases of malarial fever, with special reference to the relations existing between different types of haematozoa and different types of fever / by William Sydney Thayer and John Hewetson.

  • Thayer, William Sydney, 1864-1932.
Date:
1895
Catalogue details

Licence: Public Domain Mark

Credit: The malarial fevers of Baltimore : An analysis of 616 cases of malarial fever, with special reference to the relations existing between different types of haematozoa and different types of fever / by William Sydney Thayer and John Hewetson. Source: Wellcome Collection.

Provider: This material has been provided by the Augustus C. Long Health Sciences Library at Columbia University and Columbia University Libraries/Information Services, through the Medical Heritage Library. The original may be consulted at the the Augustus C. Long Health Sciences Library at Columbia University and Columbia University.

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    Sacharow/^'^' in studying the small hyaline bodies of aestivo- autumnal fever, advises the use of gentian violet on account of the intense color which the parasites take. Sforza '^' has used the method of Canon and Pielicke with good results. The dried specimen is fixed by being placed for from five to ten minutes in absolute alcohol. It is then allowed to remain for from six to twenty hours in the following solution at 37° C, washed in water, and mounted. The solution is as below: Concentrated aqueous solution of methylene blue 40. J per cent, solution of eosin in 75 per cent, alcohol 20. Distilled water 40. Laveran'^'' advises the following simple method: The dried speci- men is fixed in a mixture of alcohol and ether, equal parts, stained for thirty seconds iu a concentrated aqueous solution of eosin, washed in distilled water and dried. It is then stained for about thirty seconds in a concentrated aqueous solution of methylene blue, washed again, dried and mounted, either dry or in Canada balsam. Good results may be obtained by the use of haematoxylin, Manna- berg,*^^''' in particular, advising this method. His method he describes as follows : The dried specimen is allowed to float at first for about five minutes upon distilled water, dried between filter paj^er, and washed in a very dilute solution of acetic acid (acetic acid gtt. j; dis- tilled water 20 ccm.) until complete disappearance of the haemoglobin. The entirely colorless preparation is then floated for two hours upon the fixing solution : Concentrated aqueous solution of picric acid 30. Distilled water 30. Glacial acetic acid 1. From this it is placed in absolute alcohol for about two hours. This is followed by staining for twelve to twenty-four hours in alum hae- matoxylin ; then by difierentiatiou by means of 0.25 per cent. HCl alcohol (alcohol 75 per cent.) and ammonia alcohol (3 drops of ammonia to 10 ccm. 75 per cent, alcohol), washiug iu 8 per cent, alcohol, mounting in Canada balsam. The washiug of the prepara- tion with water and acetic acid causes the removal of all ali)uminous substances which would otherwise give rise to annoying precipitates