The frog : an introduction to anatomy, histology, and embryology / by the late A. Milnes Marshall ; edited by F.W. Gamble.

  • Marshall, A. Milnes (Arthur Milnes), 1852-1893.
Date:
1912
Catalogue details

Licence: In copyright

Credit: The frog : an introduction to anatomy, histology, and embryology / by the late A. Milnes Marshall ; edited by F.W. Gamble. Source: Wellcome Collection.

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    the oil of cloves. The molted paraffin should then be washed off by turpentine, when the sections will remain fixed to the slide by the collodion, :ind may be mounted in balsam in the usual manner. . Instead of the mixture of collodion and oil of cloves, a solution of shellac in absolute alcohol may be used: this should be spread over the slide in a thin layer by means of a glass rod, and allowed to dry. Immediately before being used the slide should be brushed over with oil of cloves. If it is desired to stain the sections on the slide, the thinnest possible layer of glycerine and albumen (pure glycerine and fresh white of egg in equal parts, filtered) should be smeared over the slide, and the sections laid on it in order. The paraffin, when melted, should be washed off with turpentine or benzole, and the sections dehydrated with absolute alcohol, the desired stain can then be used in the usual manner. Dehydrate with absolute alcohol, followed by oil of cloves, before mounting in Canada balsam.