Anærobic infection in animals : discussion on Professor Gaiger's paper Mr. Dalling's views.

  • Dalling, Thomas.
Date:
[1924?]
Catalogue details

Licence: In copyright

Credit: Anærobic infection in animals : discussion on Professor Gaiger's paper Mr. Dalling's views. Source: Wellcome Collection.

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    medium giving best results in our experience is Tarozzi’s, a meat broth containing 1 per cent, glucose, to which has been added a small piece of uncooked sterile guinea-pig liver. The results are most grati- fying. (b) Virulence of B. chauvoei. Mr. Gaiger states that B. chauvoei retains its viru¬ lence for years in artificial culture. I cannot agree with this statement, as our experience is exactly the reverse. Much time has been lost in some of our experimental work, because of the rapidity with which our cultures of B. chauvoei have lost their virulence. As an example of this let me quote the following :— A meat broth culture of B.chauvoei killed guinea- pigs on intramuscular inoculation in doses of 0*3 c.c. in 24—48 hours. It had been left untouched in the same culture tube for six weeks, and on retesting for virulence was found to produce death only in doses of 3 c.c. By repeated passage it has been found possible to obtain a culture having the original lethal dose for guinea-pigs. Personally, I would feel very grateful if someone could give us some enlightenment on this point. (c) Suprarenal Reaction in Vibrion septique Infection. One reads commonly in text-books and articles describing guinea-pig infection with cultures of Vibrion septique that the suprarenals are haemor¬ rhagic. The veterinary department of our laboratory have post-mortem examinations on such guinea-pigs practically every day, and I cannot call to mind this feature. Possibly it only occurs in delayed deaths or in early deaths, but our experience does not show it to occur in animals which die overnight. (d) Filaments on Peritoneal Surface of the Liver. While I agree that the presence of filaments on the peritoneal surface of an injected guinea-pig’s liver is of considerable service in the identiiication of Vibrion septique, I cannot confirm that it is absolutely diagnostic. While working with some commercial blackleg products from an antigenetic point of view many guinea-pigs had to be inoculated with the various samples. Some guinea-pigs died as a result of the inoculation, and in the case of one product filaments were present on all the dead guinea-pig