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Mouse brain, coronal view. Attribution-NonCommercial 4.0 International (CC BY-NC 4.0). Source: Wellcome Collection.
Coronal view of a section of mouse brain which has been sliced down a vertical axis to divide it into front and back. To create this image, brain tissue was first rendered optically transparent in order to be able to look deep into the tissue. Confocal microscopy was then used to visualise a subset of neurones in the brain which express green fluorescent protein (GFP). Individual images were captured every 5.3 micrometres through a 751 micrometre thick piece of tissue and then subsequently combined into the single image seen here. Neurones are colour-coded by depth from red (top) to orange, yellow, purple, blue and green (bottom) as you look into the image. Size of image is 7,352 (x) x 4,461 (y) x 751 (z) micrometres. Confocal micrograph 2014