On the leucocytes of peptone and other varieties of liquid extravascular blood / by A.E. Wright.

  • Wright, Almroth, 1861-1947.
Date:
[1893]
Catalogue details

Licence: Public Domain Mark

Credit: On the leucocytes of peptone and other varieties of liquid extravascular blood / by A.E. Wright. Source: Wellcome Collection.

Provider: This material has been provided by The University of Glasgow Library. The original may be consulted at The University of Glasgow Library.

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    results of these enumerations here, and to direct attention to the bearing of the results obtained in the,case of peptone blood. I may premise with respect to the methods employed that the enumerations were made by diluting the blood 200 times with an 8 per cent, solution of magnesium sulphate, which had received a sufficient addition of gentian-violet to stain the cells darkly in the course of a few minutes. The enumerations were invariably made in duplicate samples of each blood, and in each sample the absolute number of leucocytes was counted on 250 squares of the Gowers hffimocytometer. An agreement within 10 per cent, was exacted between the counts of the duplicate samples. When this was obtained the counts were added together, and their sum multiplied by 200 to obtaiu the total number of leucocytes in the cubic millimetre. The following are the results of my enumerations in oxalated, leech- extract, aud peptone blood. Oxalated Blood. Obtained by drawing off 9 vols, of blood into 1 vol. of a 1 per cent, oxalate of soda solution. Dog 1 Dog 7 Dog 8 Dog 14 Number of white blood corpuscles in normal blood drawn fi-om ear. Number of white blood corpuscles in blood received from carotid into oxalate solution (figures corrected for di- lution effected by the oxalate solution). Interval between collection of blood into oxalate and commencement of enumeration {i.e., dilution with MgS04 solution). 15,600 10,400 20,500 14,000 22,300 15,800 11,200 19,700 14,600 20,600 One hour. Half an hour. No interval. Two hours. One hour. 82,800 81,900 16,560 16,380 Leech-eoctract Blood. Obtained by extracting 3 vols, of blood from carotid into 2 vols, of leech-extract. The leech-extract was made from leeches which had been kept under alcohol for several weeks.