Sister chromatid exchange / editor, Avery A. Sandberg.

Date:
[1982], ©1982
Catalogue details

Licence: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

Credit: Sister chromatid exchange / editor, Avery A. Sandberg. Source: Wellcome Collection.

    78/736 (page 56)
    56 / Jenssen — ф и (О E о и >ч о а О Ф (О ф и 3 с 0 и 1 0.8 1.2 1.6 mmol per kg b. w. Fig. 3. The number of micronucleated polychromatic erythrocytes in mouse bone marrow af¬ ter MMS or DMN treatments in combination with caffeine pretreatments. The co-mutagenic ef¬ fect of caffeine was statistically significant at both doses of DMN but only at the high dose of MMS. MICRONUCLEUS INDUCTION AS A TEST SYSTEM FOR MUTAGENICITY Experimental Animals The induction of micronuclei as a test for mutagenicity can be studied in al¬ most any species. If micronuclei are studied in mammals in vivo, erythrocytes in the bone marrow constitute the most favorable material, which also is the one most used. Mice and rats are the most commonly used animals. No major difference in response between males and females has been reported with the exception of the investigation by Henry et al. [48], who observed a sex-related difference in response to EMS in mice. Experimental factors such as body tem¬ perature have been shown to affect the frequency of micronuclei [49]. No ef¬ fect of age has been found [unpublished results]. In a series of experiments over 1 year, the effect of a low dose of X-rays given at different times to aging mice did not differ from the effects in 2-month-old mice (Table 4-1).