Sister chromatid exchange / editor, Avery A. Sandberg.

Date:
[1982], ©1982
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Licence: Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)

Credit: Sister chromatid exchange / editor, Avery A. Sandberg. Source: Wellcome Collection.

    65/736 (page 43)
    SCE and DNA Lesion Persistence / 43 the same DNA lesion(s) in the same location in every cell generation, the over¬ all effect would be to negate any previous exchange event and, of course, to significantly affect the frequency of SCEs per cell generation. Since this nega¬ tion does not appear to occur, we can only surmise that while DNA lesions induce SCE, the geometric relationship between the appropriate lesion and the elicited SCE is not precise. We hope that through studies on DNA lesion persistence such as have been presented here we will not only gain more information about repair of DNA lesions induced by different DNA-damaging agents and in different types of proliferating cells but also achieve greater insight into the nature of the SCE phenomenon. ACKNOWLEDGMENTS This research was carried out at Brookhaven National Laboratory under contract with the U.S. Department of Energy, the U.S. Environmental Protec¬ tion Agency, and the National Institutes of Health. REFERENCES 1. Latt SA: Sister chromatid exchanges, indices of human chromosome damage and repair: Detection by fluorescence and induction by Mitomycin C. Proc Natl Acad Sci USA 71:3162, 1974. 2. Perry P, Evans HJ: Cytological detection of mutagen-carcinogen exposure by sister chro¬ matid exchange. Nature 258:121, 1975. 3. Nakinishi Y, Schneider EL: In vivo sister chromatid exchange: A sensitive measure of DNA damage. Mutat Res 60:329, 1979. 4. Latt SA, Allen J, Bloom SE, Carrano A, Kram D, Schneider E, Schreck R, Tice R, Whit¬ field BL, Wolff S: US EPA Gene-Tox Workshop report: Sister chromatid exchange. Mutat Res 87:17, 1981. 5. Taylor JH, Woods PS, Hughes WL: The organization and duplication of chromosomes as revealed by autoradiographic studies using tritium-labeled thymidine. Proc Natl Acad Sci USA 43:122, 1957. 6. Latt SA: MÍcrofluorometric detection of deoxyribonucleic acid replication in human meta- phase chromosomes. Proc Natl Acad Sci USA 70:3395, 1973. 7. Schvartzman JB, Tice RR: Chapter 8, this volume. 8. Kato H: Spontaneous and induced sister chromatid exchanges as revealed by the BudR- labehng method. Int Rev Cytol 49:55, 1977. 9. Taylor JH: Sister chromatid exchanges in tritium-labeled chromosomes. Genetics 43:515, 1958. 10. Tice R, Chaillet J, Schneider EL: Evidence derived from sister chromatid exchanges of restricted rejoining of chromatid subunits. Nature 256:642, 1975. 11. Wolff S, Perry P: Insight on chromosome structure from sister chromatid exchange ratios and the lack of both isolabeling and heterolabeling as determined by the FPG technique. Exp Cell Res 93:23, 1975.