Medical genetics / edited by Victor A. McKusick and Robert Claiborne.

Date:: [1973]

Catalogue details

Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Credit: Medical genetics / edited by Victor A. McKusick and Robert Claiborne. Source: Wellcome Collection.

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BACH AND BACH Cells Mitomycin С Recipient Cells 3 Incubation Tritiated Thymidine Recipient Cells with Tritiated Thymidine 6 1 In one-way mixed leukocyte culture (MLC), donor leukocytes in suspension are inactivated with mitomycin C, which blocks DNA synthesis but leaves cells' antigens intact, enabling them to stimulate other cells (1). After inactivated cells are mixed with recipient leukocytes not so treated (2), the cells are cultured to¬ gether for three days (3);tritiated thymidine is then added (4) 186 and is subsequently incorporated into DNA of the recipient cells to the extent these are stimulated by the donor cells — i.e., to the extent donor cell antigens are not present on recipient cells. After a set period of time, cells are filtered and washed (5); stimula¬ tion is then measured by the radioactivity in the precipitate, which represents amount of thymidine taken up by recipient cells.