A manual of bacteriology, clinical and applied / by Richard T. Hewlett.

  • Hewlett, R. Tanner (Richard Tanner), 1865-1940.
Date:
1902
Catalogue details

Licence: Public Domain Mark

Credit: A manual of bacteriology, clinical and applied / by Richard T. Hewlett. Source: Wellcome Collection.

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    5. Treat with distilled water containing a trace of ammonia. The sections remain in this until they assume a deep blue colour. 6. They can then be dehydrated, cleared and mounted, or counter-stained with eosin or orange-rubin and then mounted. Hsematoxylin makes a good contrast stain for the tubercle and the leprosy bacillus and for actinomycosis. 12. Ehrlich-Biondi Triple Stain. This is best bought ready for use. It is a good histological stain for tissues and leucocytes. Actinomycosis stains well by it. Stain for ten to sixty minutes, then treat with methylated spirit until the section becomes greenish. Pass through abso¬ lute alcohol, clear, and mount. 13. Jenner’s Blood-Stain.1 The precipitate formed by mixing aqueous solutions of eosin and methylene-blue purified and dissolved in methyl-alcohol. It is best bought ready prepared. An excellent double stain for blood-films and smear preparations, but not very permanent. Bacteria and nuclei are stained blue, other structures pink. It brings out the granulation of the leucocytes. Films are stained for two minutes, evaporation being prevented, and are then thoroughly washed in distilled water. 14. Beale’s Carmine. Carmine (best) . Liq. Ammon. Fort. Pure glycerin 10 grains \ drachm 2 ounces Alcohol ..... ^ ounce Distilled water .... 2 ounces Used for staining fresh tissues and protozoa. Eosin, orange-rubin, hgematoxylin, picrocarmine, and Beale’s carmine keep well in solution ; the remainder may or may not, and are best used fairly fresh. All stains should be filtered before use, and may be conveniently kept in bottles having a 1 Lancet, 1899. i. p. 870.