Elementary practical biology vegetable / by Thomas W. Shore.

  • Shore, Thomas W.
Date:
1887
Catalogue details

Licence: Public Domain Mark

Credit: Elementary practical biology vegetable / by Thomas W. Shore. Source: Wellcome Collection.

    18/206 (page 6)
    Cover-glasseti, of very thiu glass, about | inch in diameter. A Section Lifter, consisting of small thin plate of metal provided with a handle. A Needle. And some suitable Mounting Solution. When it is desired simply to examine a tissue in its fresh state, sections or objects can be mounted in water. But, as a rule, the medium employed in these exercises is glycerin, or glycerin-jelly. In mounting in glycerin, take a drojD on the middle of a glass slide, and then transfer the section from spirit, in a watch-glass, to the slide, by means of a section lifter. Then carefully drop over the specimen a thin cover-glass. If it is desired to keep the section permanently, the cover-glass should be painted round with Canada balsam or some other cement. Specimens which have been cleared by means of potash should be mounted in glycerin. Sections stained in logwood may be mounted either in glycerin or in Canada balsam. If in the latter, they must be previously cleared by the clove oil process. Those stained in carmine may be mounted in the same way. Sections stained in the aniline dyes are usually mounted in Canada balsam. In all cases, if sections are mounted in balsam, they must he cleared by the clove oil method. In the following exercises, unless d.irections are given to the contrary, the specimens should be examined by mounting in glycerin. PREPARATION OF REAGENTS. 1. Pasteur’s Solution. Ammonium Tartrate Cane Sugar Potassium Phosphate Calcium Phosphate Magnesium Sulphate Water ... •2 •2 837-6 parts. 1000-0 „ Iodine Solution. Dissolve Iodine and Iodide of Potassium in distilled water, and dilute with water till the solution is of a dark sherry colour.