Using the laboratory : a handbook for medical practitioners / prepared on the advice of the Standing Medical Advisory Committee and the Central Pathology Committee.

  • Great Britain. Standing Medical Advisory Committee.
Date:
[1971]
Catalogue details

Licence: Open Government Licence

Credit: Using the laboratory : a handbook for medical practitioners / prepared on the advice of the Standing Medical Advisory Committee and the Central Pathology Committee. Source: Wellcome Collection.

    92/108 (page 90)
    (10% ferric chloride in 2N HCl). A plum coloured solution indicates acetoacetic acid. Salicylates and p-amino salicylic acid give violet colours often difficult to distinguish from acetoacetic acid. ‘Acetest’ or ‘Ketostix’ can be used to differentiate because they are specific for Ketones, or a further sample of urine can be boiled for 10 minutes in an open dish when acetoacetic acid will decompose. Phenylpyruvic acid, and some other phenolic compounds, give green colours and some drugs give other colours, eg. chlorpromazine (mauve). In the absence of drugs a positive test is pathognomonic of severe ketosis. (b) Acetest (Ames Co). A tablet test similar to but less sensitive than Rothera’s test. Use according to maker's instructions. ‘Ketostix” (Section 9.1.3 Ames Co) is based on the same principle. 9.2.6 Bile Pigments in Urine (a) Bilirubin. Ictotest (Ames Co). Aspecific diazo tablet test. Use according to the maker's instructions. The limit of detection is about 0.1mg/100mI. (b) Urobilinogen (or Porphobilinogen). Watson and Schwartz modification of Ehrlich’s test. To 1 ml fresh urine add 1 ml reagent. (0.7g p-dimethylaminobenzaldehyde in a mixture of 150ml