Upon an anti-cholera serum / by Allan Macfadyen.

  • Macfayden, Allan, 1860-1907.
Date:
[1906]
Catalogue details

Licence: In copyright

Credit: Upon an anti-cholera serum / by Allan Macfadyen. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    ascertained lethal doses. The serum-toxin mixture was kept at 37° C. for half an hour before injection into the guinea- pig. The following table represents the results obtained. Serum Jests. Goat. Elood sample. Ascertained lethal doses of toxin. Amount ot serum. Guinea-pig. Result. After 6 injections. 3 ID C.C. Alive. 1* ,!» 11 3 c.c. 11 It 11 11 3 ED C.C. 11 II 13 5 ID C.C. It 11 II II 5 C.C. 11 11 14 3 C.C. 11 It 11 11 3 ID C C. li It 11 11 3 ED C.C. 11 It 11 11 3 ED C.C. 11 11 11 II 3 1DD C.C. 1 * 11 11 11 3 IDD C.C. 11 15 3 iJd c.c. 11 11 li 11 3 Sou C.C. 11 11 11 11 3 EDD C.C. ♦ 1 11 11 li 3 jin C.C. 11 11 11 11 3 EOD C.C. 11 11 3 EDD C.C. 11 The figures demonstrate that marked anti-endotoxic properties had developed in the serum of the goat and had increased to the degree that sj^th cubic centimetre of the serum neutralised three ascertained lethal doses of the endotoxin, a property not possessed by one cubic centimetre of normal serum. A few tests were made with the goat’s serum on the rabbit and showed that the serum likewise acted on intravenous injection. For example, 5Vfcli cubic centimetre of the serum protected a rabbit against the intravenous injection of from three to four lethal doses of the endotoxin, and in other tests similar results were obtained. The experiments having served their purpose have not in the meanwhile been carried farther, although I have little doubt that still higher serum titrates could have been obtained. They demonstrate not only the feasibility of pro- ducing an antibody for the cholera endotoxin by the method