Molecular cloning and analysis of lymphokines / edited by David R. Webb, David V. Goeddel.

Date:
1987
Catalogue details

Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Credit: Molecular cloning and analysis of lymphokines / edited by David R. Webb, David V. Goeddel. Source: Wellcome Collection.

    9/344 page V
    Contents Preface xi Strategies for Cloning Mouse and Human Lymphokine Genes Using a Mammalian cDNA Expression Vector Takashi Yokota, Frank Lee, Naoko Arai, Donna Rennick, Albert Zlotnik, Tim Mosmann, Atsushi Miyajima, Yutaka Takebe, Robert Kastelein, Gerard Zurawski, and Ken-ichi Arai I. Molecular Biology of T Cell-Derived Lymphokines 1 II. Isolation of Lymphokine cDNA Clones Using pcD Mammalian Expression Vector 2 III. Expression of Lymphokine cDNA Clones in Various Host-Vector Systems 12 IV. Biological Characterization of Recombinant Lymphokines 15 References 17 Identification and Expression of cDNA Plasmids Corresponding to Human Interferon-Gamma (IFN-7) and Interleukin 2 (IL-2) Rene Devos, Hilde Cheroutre, Geert Plaetinck, and Walter Fiers 1. Introduction 21 II. Bioassay for Human IFN-7 and IL-2 22 III. Preparation of Human Spleen Cell Cultures and Induction of IFN-7 and IL-2 22 IV. Detection of mRNA Corresponding to Human IFN-7 and IL-2 .. 23 V. Identification of cDNA Plasmids Corresponding to Human IFN-7 and IL-2 24 VI. Expression of Human IFN-7 and IL-2 30 VII. Biological Studies Using Recombinant IFN-7 and IL-2 32 References 33 V
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