The dessication of serum and other protein solutions : with special reference to the reagents used in the Wassermann Reaction / by P. Hartley, A.J. Eagleton, and C.C. Okell.

  • Percival Horton-Smith Hartley
Date:
[1923?]
Catalogue details

Licence: In copyright

Credit: The dessication of serum and other protein solutions : with special reference to the reagents used in the Wassermann Reaction / by P. Hartley, A.J. Eagleton, and C.C. Okell. Source: Wellcome Collection.

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    Equivalent of fresh guinea-pig serum (c.c.) for ten to fifteen minutes and then the whole was stirred gently with a very thin glass rod until solution was complete. The results of this experiment are shown in the following chart. During the first two months there is little change in activity. Deterioration then sets in and at the end of four months the value is reduced to about one half. It is interesting to note, however, that with this particular sample of dried complement there was no further decline in value during the next five months. This result has been obtained on other occasions. Thus, another sample of dried complement, H = Titre of original guinea-pig serum (undried.) (7) = Titre of reconstructed dried serum on the dates shown. Total volume in each tube = 3 c.c.; 0-5 c.c. 5 per cent, red-blood cells. Chart II.—Illustrating stability of dried complement. the original titre of which was 0-015 c.c., had a value of 0-032 c.c. after being kept—partly at room temperature and partly in the cold room— for 9J months. It is also of interest to record that another sample of dried complement, sent to Uganda, was successfully used there for the purpose of the Wassermann reaction. It is possible that the deteriora¬ tion which occurs is due to traces of moisture which the dried serum contains, and that if the moisture content be further reduced the dried complement may be found to be even more stable. Further, although in six months the activity falls to one-half, yet even then the dried complement when suitably reconstructed is still serviceable. The dried
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