A manual of elementary microscopical manipulation for the use of amateurs / by T. Charters White.

  • White, T. Charters (Thomas Charters), 1828-1916.
Date:
1887
Catalogue details

Licence: Public Domain Mark

Credit: A manual of elementary microscopical manipulation for the use of amateurs / by T. Charters White. Source: Wellcome Collection.

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    ployed, and for this the student must be referred to the bibliography. While on the subject of staining, it may be interesting to direct the beginners’ attention to the beauty of some vegetable sections, if double stained, and as this does not entail much difficulty in its accomplishment, it will be an addition to his pleasure if directions are here given whereby he can make them. Judson’s scarlet dye may be added to water till a good colour is obtained, when the solution must be filtered through blotting paper. Judson’s blue dye may be dis- solved in methylated alcohol, and the solution filtered. These solutions, well corked, keep for a considerable time. Prepare two watch-glasses, and put a little of the red solu- tion in one, in which place the section. When it becomes stained an uniform red, remove it to the other watch-glass, in which pour some of the blue. This will drive out the red stain from all but those definite portions of the section to which the red will cling with great tenacity; after which, it may be treated according to the foregoing directions, and mounted permanently. Public attention has been frequently directed to the subject of bacilli, and their occurrence in the sputa <>i phthisical patients, and a chapter devoted to staining would be incomplete without reference to a method of staining bacilli, which seems to give good results all round. The sputum is spread in a thin layer on a cover-glass, and dried at a temperature of 919 -230 , or passed three or four times through the flame of a spirit lamp. The stain- ing fluid is prepared as follows : 5 c.c. pure aniline added to 100 c.c. of distilled water, well shaken, and then filtered through moistened blotting paper. To this mixture a saturated alcoholic solution of fuchsine, methyl violet, or gentian violet is added, till precipitation commences. The cover glass is allowed to float on this with the sputum downwards for from a quarter to half hour. It is then washed for a few seconds in nitric acid, one part, distilled