The significance of the supra-renal capsules in the action of certain alkaloids / by H.H. Dale and P.P. Laidlaw.

  • Dale, Henry Hallett, 1875-1968.
Date:
1912
Catalogue details

Licence: In copyright

Credit: The significance of the supra-renal capsules in the action of certain alkaloids / by H.H. Dale and P.P. Laidlaw. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    necessary to show, therefore, that the increase of adreriiue content was relatively greater than the decrease in rate of flow. The following are the details of the experiments. It will be seen that an accurate standardisation of the adrenine content was made in one case only. In others it was considered sufficient to show that the increase of adrenine was more than could be accounted for by diminished perfusion. Exp. 1. Eate of flow from the vena cava slow. (Unmixed supra-renal blood.) (A) Normal sample—3 c.c. collected in 45. (B) Sample 1' after 4 mgms. pilocarpine—15 c.c. collected in 128. Normal rate _ 128 x 2 _ ^ Pilocarpine rate 45 The samples were tested on an isolated non-pregnant uterus in 20 c.c. of Ringer’s solution. 0T c.c. of B produced a relaxation of the uterus approximately equal to that produced by 0-1 c.c. of 1:100,000 adrenine. (Dilution in bath 1 in 20 millions.) 0T c.c. of A caused a scarcely perceptible relaxation. 0-5 c.c. of A produced a moderate effect. 0 05 c.c. of B produced a deeper relaxation than that caused by 0-5 c.c. of A, but less than that produced by 0-75 c.c. of A. (Fig. 6.) Fig. 6. Testing of supra-renal blood on isolated non-pregnant cat’s uterus. See text (Exp. 1). The adrenine content of B was, therefore, more than 10 times, but less than 15 times that of A—say 12 times. We have, then, rates of flow as 5’7 : 1, adrenine content as 1 : 12. Clearly the rate of output of adrenine has been at least doubled by the pilocarpine injection. During the effect of pilocarpine the blood from the supra-renal vein, entering the general circulation at the rate of about 1 c.c. in 85, contains about 0 01 mgm. adrenine per c.c.