The effect of L-triiodothyronine on the growth and development of embryonic chick limb-bones in tissue culture / by Honor B. Fell and E. Mellanby.

  • Fell, Honor B.
Date:
[1956?]
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Licence: In copyright

Credit: The effect of L-triiodothyronine on the growth and development of embryonic chick limb-bones in tissue culture / by Honor B. Fell and E. Mellanby. Source: Wellcome Collection.

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    Heming, A. E. & Holtkamp, D. E. (1953). Calorigenic and antigoitrogenic actions of L-triiodo- thyronine and L-thyroxine in thyroidectomised and intact rats. Proc. Soc. exp. Biol., N.Y., 83 (4), 875-879. Kaltenbach, J. C. (1953). Local action of thyroxine on amphibian metamorphosis. 1. Local metamorphosis in Rana pipiens larvae effected by thyroxincholestrol implants. J. exp. Zool. 122, 21-36. Lebman, J. (1953). The physiologic activity of L-triiodothyronine. J. din. Endocrin. 13, (11), 1341-1346. Rawson, R. W., Rall, J. E., Peabson, 0. H., Robbins, J., Poppell, H. F. & West, C. D. (1953). L-triiodothyronine versus L-thyroxine. A comparison of their metabolic effects in human myxoedema. Trans. Ass. Amer. Phycns, 66, 86-94. Richteb, K. M. (1944). Some new observations bearing on the effect of hyperthyroidism on genital structure and function. J. Morph. 74, 375-387. Shellababgeb, C. J. & Godwin, J. T. (1954). Effects of triiodothyronine on tadpoles. Endo¬ crinology, 54 (2), 230-232. Staeb, P. & Liebhold-Shoeck, R. (1953). Effect of oral thyroxine and triiodothyronine on radioactive iodine uptake and serum protein bound iodine in normal human subjects. Proc. Soc. exp. Biol., N.Y., 83, 52-54. Tomich, E. G. & Woollett, E. A. (1954). Relative activities of triiodothyronine and thyroxine. J. Endocrin. 11 (2), 131-141. EXPLANATION OF PLATES Photographs by Mr V. C. Norfield, Strangeways Research Laboratory. All the sections were stained with Delafield’s haematoxylin and chromotrop 2 R. ep., epiphysis; g.z., growing zone; z.li.c., zone of hypertrophic cells. Plate 1 Fig. 1. a : femur from an embryo of group 3, grown for 4 days in control medium (C); b: opposite femur from the same chick grown in medium containing TIT 15-6/^g/lOO ml. The epiphyses are larger and the growing zones narrower in C than in TIT; the hypertrophic zones are about the same size. Fig. 2. Humeri from the same embryo as the femora in Fig. 1 after 4 days’ growth (a) in C, (b) in TIT. The epiphyses are larger, the hypertrophic zone slightly longer and better developed and the growing zones narrower in TIT than in C. Fig. 3. Femora from an embryo of group 3, after 8 days’ growth (a) in C, (b) in TIT. Note the great reduction of the growing zones in TIT. Fig. 4. Radii from an embryo of group 3, after 8 days’ cultivation (a) in C, (b) in TIT. Differentia¬ tion has been suppressed in C and the explant has produced only a nodule of unossified, small - celled cartilage, whereas the radius in TIT has formed hypertrophic cartilage, growing zones and epiphyses in the normal way. Plate 2 Fig. 5. Comparable areas in the proximal epiphyses of the femora shown in Fig. 1. (a) in C, (b) in TIT. The cartilage is better developed in TIT than in C; the cells are larger and the matrix is more profuse. Fig. 6. The proximal ends of the humeri shown in PI. 1, fig. 2 (a) in C, (6) in TIT. The epiphysis is larger and the growing zone is narrower in TIT than in C. Fig. 7. The proximal ends of the femora from an embryo of group 4, after 8 days’ cultivation (a) in C, (b) in TIT. The growing zone is greatly reduced in TIT.
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