The cerebellar cortex / by C.E. Beevor.

  • Beevor, Charles, E. (Charles Edward), 1854-1908.
Date:
1883
Catalogue details

Licence: Public Domain Mark

Credit: The cerebellar cortex / by C.E. Beevor. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    THE CEEEBELLAR CORTEX.* BY C. E. BEEVOK, M.D. BOND., M.R.C.P. Assistant riiysician to the National Hospital for Paralysed and Epileptic, London. Dr. Gaule, under whom I liave worked, and whom I have much to thank for liis kind assistance, advised me to iise Wei^ert’s saure fuchsia method (Centrhl. fur die med. ITm. 1882, p. 753), wliich has produced excellent results. The first sections weie made from pieces of cerebellum, one centim. square, hardened in three per cent, soluiion of bichromate of potash and kept in a hot chamber at 35° C. for 4-8 days. They were then washed in water half an hour, then ])laced in alcohol 24 liours, and then for 24-28 hours in a concentrated aqueous solution of siiuie fuchsin, also at a temperature of 35° C. The pieces were then slightly washed in water and put into .strong alcohol and dehy- drated, imbedded in paraffin and cut into section.s, which were then fixed to object slides, the paraffin dissolved (mt,'-* and the sections washed out according to Weigert’s method with water and alkaline alcohol. In the second series of preparations they were further stained with nigrosine after the above treatment; and these preparations proved the best. The third series were hardened in the same way, but after fixing the sections on the slides, they were stained by hajmatoxylin and eocene. This latter method of double staining has alread}' been employed by Denissenko. In the fourth method employed, the pieces of cerebellum were hardened by % chiomic acid, imbedded unstained, and the sections fixed on the object slide. The specimens were taken from the cerebellum of man (adult and new-born) dog, rat, rabbit, hen and pigeon. My des- cription is chiefly concerned with the dog, in which all the elements are most clearly shown. The human cerebellar cortex is the most developed, and as it is much richer in medullated fibres, and the inedullated sheaths are better developed, it would undoubtedly give better preparations, but unfortunately it is impossible to harden the pieces directly after death and so obviate post-mortem changes. And great stress is laid on the fact that in the preparations used for this paper, in less ihan an hour after death, the pieces were in the hardening fiuid in the warm chamber, which was used to introduce a quicker diffusion in the substance of the piece. ’ This article is an extract from a paper published in the ‘ Archiv fiir Anatomie und Physiologie’ (Physiologische Abtheilung) and from work done in Professor Ludwig’s Physiological Institute in Leipzig. * Canini, in these Archive, 188R, p. 155. B 2