Studies on the proteids of rye and barley and on the chemical nature of diastase.

  • Osborne, Thomas B. (Thomas Burr), 1859-1929.
Date:
[1893]
Catalogue details

Licence: Public Domain Mark

Credit: Studies on the proteids of rye and barley and on the chemical nature of diastase. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    Preparation 6. Ash-free. 53.11 6.45 15.78 Sulphur 1 24.66 Oxygen ) 100.00 Ash 0.75 The filtrate from preparation 6 still contained proteid matter which was separated by dialysis in alcohol. Preparation 7 was so obtained, weighing 1.54 grams, having the same properties as 6, and the following similar composition : Preparation 7. Ash-free. Carbon 53.58 Hydrogen 6,70 Nitrogen 15.87 Sulphur I 23 35 Oxygen ) 100.00 Ash 1.43 After extracting precipitate I with water ftnd salt solution a very considerable part still remained undissolved. This was treated with water to remove all the salt, and then with alcohol, and was dried over sulphuric acid. This preparation, 8^ weighed 8 grams and was quite dark in color. It had the properties of an insoluble form of globulin, being dissolved in one-half per cent, sodium carbonate solution and precipitated therefrom by neutrali- zation. Its composition was nearly the same as that of the two last globulin-like preparations and is probably a so-called “ albu- minate ” derived from that substance. The composition of prepa- ration 8 was: Preparation 8. Ash-free. Carbou 53.55 Hydrogen 7.01 Nitrogen 15.72 Sulphur 1.23 - Oxygen 22.49 100.00 ■A-Sh 1.09 Precipitate II was treated with water and the solution thus formed was dialyzed in water for several days and then in alcohol