Guide to the microscopic examination of the eye / by R. Greeff ; translated from the second German edition by Hugh Walker.

  • Greeff, R. (Richard), 1862-1938.
Date:
1901
Catalogue details

Licence: In copyright

Credit: Guide to the microscopic examination of the eye / by R. Greeff ; translated from the second German edition by Hugh Walker. Source: Wellcome Collection.

Provider: This material has been provided by UCL Library Services. The original may be consulted at UCL (University College London)

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    Carmine. Carmine is one of the oldest and most popular of stains. It is principally a nuclear stain, but it also tinges the protoplasm of the cell body. It acts more slowly than haematoxyhn ; its solutions keep better, and are not so sensitive to acids. For tissues which are difficult to stain hasmatoxylin is preferable. Only one or two of the following solutions need be kept: — 1. Alum-Carmine or Grenacher s Carinine.—This stains the nuclei cherry-red, and is almost purely a nu- clear stain. Overstaining does not readily occur. 2-5 grammes of powdered carmine are boiled for yi-i hour with looc.cm. of a 5 per cent, solution of- ammonia-alum. The solution is filtered when cool. For use 3 or 4 c.cm. are filtered into a watch-glass. Staining, 10 minutes to 2 hours. Washing in water for a few minutes. 2. Borax-Carmine (alcoholic solution).—3 grammes of carmine and 4 grammes of borax are dissolved in 93 c.cm. of water. The solution is allowed to stand for I or 2 days, and then mixed with 100 c.cm. of 70 per cent, alcohol. The mixture is again set aside for several days, stirred occasionally, and finally is filtered. Since this is an alcoholic solution, the sections are transferred to it from the 70 or 80 per cent alcohol in which they are preserved, without previous im- mersion in water. Staining for hour or longer. Overstaining does not occur.