Guide to the microscopic examination of the eye / by R. Greeff ; translated from the second German edition by Hugh Walker.

  • Greeff, R. (Richard), 1862-1938.
Date:
1901
Catalogue details

Licence: In copyright

Credit: Guide to the microscopic examination of the eye / by R. Greeff ; translated from the second German edition by Hugh Walker. Source: Wellcome Collection.

Provider: This material has been provided by UCL Library Services. The original may be consulted at UCL (University College London)

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    tions remain in this till they become faint rose-red (1-5 minutes). They are rinsed in water containing a trace of eosin. Eosin, unlike h?ematoxylin, is very feebly held by the tissues and is immediately extracted by liquids which do not already contain some of it. Hence a trace of it is added to the water and to the various alcohols with which the sections are treated. Alcohol-(eosin), 80 per cent. Alcohol-(eosin),. 96 per cent. Oil, or carbol-xylol and xylol. Canada balsam. The contrast staining can also be carried out in alcohol. A few drops of the concentrated aqueous solution of eosin are added to the 80 per cent, alcohol, and the sections are stained in this. Then they are transferred to stronger alcohol, oil, Canada balsam. The following stains have been recommended in place of eosin, and yield even more beautiful results : — 2. Orange G. — An anilin dye. A concentrated aqueous solution is prepared, and used diluted with water or alcohol. 3. Aurantia is employed in the same manner. 4. Acid-Fuchsin.—This is used like the others of tlie series, but, unlike them, is not removed by wash- ing, so that care must be taken not to overstain. Triple Staining. I. Va7i Giesons Stain :— 1. Hardening in Miiller's fluid or otherwise. 2. Imbedding in celloidin. Section-cutting. 3. Preliminary staining in Delafield's or Boehmer's