Guide to the microscopic examination of the eye / by R. Greeff ; translated from the second German edition by Hugh Walker.

  • Greeff, R. (Richard), 1862-1938.
Date:
1901
Catalogue details

Licence: In copyright

Credit: Guide to the microscopic examination of the eye / by R. Greeff ; translated from the second German edition by Hugh Walker. Source: Wellcome Collection.

Provider: This material has been provided by UCL Library Services. The original may be consulted at UCL (University College London)

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    the lirst place, it is ready for immediate use; secondly, it acts very quickly; and thirdly, overstaining is not apt to occur. Hasmalaun is, therefore, a favourite stain in some laboratories. 4. Ehrlick's Acid - Hcematoxylm. — This consists of: — Water Absolute alcohol Glycerine Glacial acetic acid Haematoxylin Alum in excess. 100 100 100 10 2 The mixture is filtered, and ripened by exposure to light until it acquires a dark red colour. It is said to keep for years, if preserved in a well-stoppered bottle. Heidenhains Iron HcEinatoxylin.—See Demon- stration of the Karyomitotic Figures. Bendds Sulphate of Iron Hcematoxylin.—See do. Weigert's Hceiriatoxylin.—This is not a nuclear stain. It is employed to demonstrate the medullary sheaths. Treatment of Overstained Sections. Sections which have been overstained in haema- toxylin and so become too dark can be cleared up by various methods : ■— 1. The stain may be partially redissolved hi a i per cent, solution of ammonia-alum. The section remains in this for several hours, till the blue colour becomes bright violet, and is then thoroughly washed. 2. The depth of the stain may be reduced by placing