Clinical diagnostic bacteriology : including serum diagnosis & cytodiagnosis / by Alfred C. Coles.

  • Coles Alfred C (Alfred Charles)
Date:
1904
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Licence: In copyright

Credit: Clinical diagnostic bacteriology : including serum diagnosis & cytodiagnosis / by Alfred C. Coles. Source: Wellcome Collection.

Provider: This material has been provided by the Royal College of Physicians of Edinburgh. The original may be consulted at the Royal College of Physicians of Edinburgh.

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    Cladius’ method gives the same result as that of Gram, with the exception that the bacillus of malig- nant oedema and symptomatic anthrax, are both stained by the former and not by the latter method. Weigert’s Modification of Gram’s Method. Film preparations are treated in exactly the same way as in Gram’s method, except that after the action of iodine, instead of being decolourised in alcohol, they are washed in xylol, then dried and after-stained with dilute carbol-fuchsin (i in io). They are again washed, dried, and mounted in oil or balsam. The following Organisms are Stained by Gram’s Method. The Bacillus of anthrax, tubercle, leprosy, tetanus, diphtheria, strepto- and staphylo-cocci pyogenes, micro coccus tetragenus, diplococcus pneumoniae (Frankel), the bacillus of rhinoscleroma, mouse septicaemia, and the mycelium of actinomycosis. The following Organisms are Not Stained by Gram’s Method. Bacillus pneumoniae (Friedliinder), influenza, plague, gonococcus, meningococcus, spirillum of relapsing fever, cholera and fowl cholera, bacillus of typhoid, coli com- munis, glanders, Malta fever, and the bacillus of soft sore. The clubs in human actinomycosis are not stained ; those in animals are. It is hardly necessary to add that a '/,2 inch oil immersion is essential for bacteriological work. I his need not be an apochromatic, as a good achromatic lens will answer all purposes, and I can most strongly recommend Leitz’ 1jn inch objective.