Hydrolysis of amandin from the almond / by Thomas B. Osborne and S.H. Clapp.

  • Osborne, Thomas B. (Thomas Burr), 1859-1929.
Date:
[1908]
Catalogue details

Licence: In copyright

Credit: Hydrolysis of amandin from the almond / by Thomas B. Osborne and S.H. Clapp. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    Copper, 0.1381 gm. subst., gave 0.0396 gm. CuO. Nitrogen, 0.2100 gm. subst., required 1.12 c.c. 5/7 N—HC1. Calculated for C4H504NCu 4^ H20 = Cu 23.07 ; N 5.08 per cent. Found = Cu 22.91 ; N 5.33 “ “ The aspartic acid from the barium salt reddened but did not decompose at 300°. Carbon and hydrogen, 0.2600 gm. subst., gave 0.3458 gm. C02 and 0.1292 gm. H,0. Calculated for C4H704N = C 36.09 ; H 5.26 per cent. Found .... = C 36.26 ; H 5.51 “ “ Fraction vi. — The aqueous layer of this fraction, remaining after the removal of phenylalanine ester with ether, was saponified with baryta in the usual way. After prolonged standing no barium aspartate had separated. The barium was accordingly removed and the concentrated solution separated 14.16 gm. of pure glutaminic acid. The decomposition point was at about I98°-I99°. Carbon and hydrogen, 0.1840 gm. subst., gave 0.2759 gm. C02 and 0.1064 gm. H20. Calculated for C5H904N = C 40.81 ; H 6.12 per cent. Found .... = C 40.89 ; H 6.42 “ “ There were further obtained from this fraction 3.4 gm. of glutaminic acid hydrochloride, while no copper aspartate could be obtained. Tyrosine. Fifty grams of amandin, moisture and ash free, were boiled with a mixture of 150 gm. sulphuric acid and 300 c.c. of water for ten hours. After removing the sulphuric acid with an equivalent quan- tity of baryta, the filtrate and washings were concentrated to small volume, and the crystalline substance that separated oh standing was filtered out, washed well with water, and recrystallized. The tyrosine thus obtained in characteristic needles weighed 0.5635 gm. equal to 1.12 per cent. The solution from which the tyrosine separated, together with the mother liquor from the recrystallizatton, was concentrated and a second separation obtained. This was dried and treated with