The blood, how to examine and diagnose its diseases.

  • Coles, Alfred Charles.
Date:
1898
Catalogue details

Licence: Public Domain Mark

Credit: The blood, how to examine and diagnose its diseases. Source: Wellcome Collection.

Provider: This material has been provided by The University of Leeds Library. The original may be consulted at The University of Leeds Library.

    46/318 (page 30)
    After trying all the usually-described methods of double staining with eosine and methylene-blue, either separately or combined in mixture, e.g., Plehn's* or Canon's, t I have never succeeded sufficiently to re- commend these stains for clinical use. No doubt the results depend very largely on the time the film^ is subjected to the action of the methylene-blue solution, and particularly on the rapidity with which the films are dried after being stained. EhrlicEs new Triple Stain seems to be very generally used. It consists of the following formula. Saturated watery solutions of orange G. acid fuchsin, and methyl- green are first prepared Saturated watery solution of Orange G 24-27 cc. 16-33 » 25 J) 60 „ 40 » 20 ,, Acid fuchsin . Methyl-green . Then add—Water . Absolute alcohol Glycerine The mixture should stand for one or two weeks before being used. Films fixed by heating or prolonged (twelve hours) action of the alcohol and ether mixture are said to give the best results. Stengel found that as a rule a rather larger quantity of methyl-green was required. * Plehn's, or Czenzinsky's Mixture, has the following formula:- Methylene-blue concentrated aqueous solution . 40 parts Eosine, i% solution in 70% alcohol • . • 20 „ Distilled water . • ■ , . . ' J\ + Canon's solution, very closely resembling this, is ^°^P^«;^ °J Methylene-blue concentrated aqueous solution . 40 paits Eosine, J% solution in 70% alcohol . ' ' ^° Fil™°™eD-,7atTons fixed by alcohol are stained in either of these mi«»es for severJhou: 3-6 accLding to MUller) rinsed in water, dr.ed, and mounted in Canada balsam.