The proteids of serum / by W.D. Halliburton.

  • Halliburton, W. D. (William Dobinson), 1860-1931.
Date:
[1884?]
Catalogue details

Licence: Public Domain Mark

Credit: The proteids of serum / by W.D. Halliburton. Source: Wellcome Collection.

Provider: This material has been provided by The Royal College of Surgeons of England. The original may be consulted at The Royal College of Surgeons of England.

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    which the most striking was the following: he collected this precipitate which occurred at 40° C. and found that it was soluble by the addition of water, from which solution it was again precipitable by saturating with magnesium sulphate. It is also possible to explain Fredericq’s results by supposing that he had too much free acid present, but on the whole Professor Schafer’s explanation seems the more reasonable. It is confirmed in a striking manner by some observations of Hammarsten^ on the action of sodium chloride on serum. One of the results of Ham mars ten’s experiments was that sodium chloride only precipitates serum globulin incompletely, not completely as Schmidt^ had previously stated. After having made this statement, and supported it by experiments, Hammarsten continues as follows:—“The amount of the precipitation of the paraglobulin from the serum by excess of finely powdered salt depends very much on the temperature. “With sodium chloride added to complete saturation, at a temperature of 17°-20° the filtered blood serum always becomes cloudy by warming it to 37°-40°C., and if this fluid is cooled it becomes gradually clear again.” “ Perfectly clear horse serum was saturated with sodium chloride: the clear filtrate concentrated in vacuo till an abundant quantity of salt had crystallised: then it was again filtered, and the filtrate heated pretty quickly to 40° C. At 30° C. the fluid became opalescent, and by 37*5° C. there was an abundant flocculent precipitate. The fluid was then placed in a cold chamber, and after three hours the precipitate had completely redissolved. It was again precipitated by heating the liquid to 37*5° C.” Hammarsten then gives details of a similar experiment in which he saturated the serum with sodium chloride at 10° C. and obtained a precipitate by heating the filtrate to 18° C. He goes on to say that there is no doubt that this precipitate is serum globulin; he washed it with salt solution at a temperature of 40° C. and dissolved it by adding water. In this fluid he got a precipitate either by saturating it with magnesium sulphate or by dialysis. Hammarsten also insists upon the difficulty of completely saturating serum with sulphate of magnesia, and upon the importance of arriving at complete saturation in order to obtain a complete precipitation of the globulin. 1 Hammarsten. “Uobcr das Paraglobulin. Erster Abscbnitt.” Pjluger's Arcliiv Bd. 17, 1878, pp. 424—426. 2 Alex. Schmidt. “ Untersuchung des Eierweisses nnd des Blutserums durch Dialyse.” Ludwig’s Juhelbnjid, pp. 99—100.