DNA sequencing protocols / edited by Hugh G. Griffin and Annette M. Griffin.

Totowa, N.J. : Humana Press, [1993], ©1993.

xii, 392 pages : illustrations ; 23 cm.

Copy 1. Donor: Wellcome Trust Sanger Institute.

DNA sequencing / Hugh G. Griffin and Annette M. Griffin -- M13 cloning vehicles : their contribution to DNA sequencing / Joachim Messing -- Cloning into M13 / Quinzhong Yu -- Transfection of E. coli with M13 DNA / Fiona M. Tomley -- M13 phage growth and single-strand DNA preparation / Fiona M. Tomley -- M13 phage growth and DNA purification using 96 well microtiter trays / Alan T. Bankier -- Generation of random fragments by sonication / Alan T. Bankier -- Generation of a nested set of deletions using exonuclease III / George Murphy -- Sequential deletions of single-stranded DNA : cyclone sequencing / George Murphy -- Subcloning for DNA sequencing / Gary M. Studnicka [and others] -- Sequencing using custom designed oligonucleotides / Ulrike Gerischer and Peter Durre -- Dideoxy sequencing reactions using Klenow fragment DNA polymerase I / Alan T. Bankier -- Dideoxy sequencing reactions using T7 polymerase / Peter Dürre and Ulrike Gerischer -- Dideoxy sequencing reactions using sequenase version 2.0 / Hugh G. Griffin and Annette M. Griffin -- Dideoxy sequencing reactions using Taq polymerase / Fabrizio Arigoni and P. Alexandre Kaminski -- Pouring linear and buffer-gradient sequencing gels / Paul Littlebury -- Electrophoresis of sequence reaction samples / Alan T. Bankier -- Plasmid sequencing / Hugh G. Griffin and Annette M. Griffin -- Plasmid sequencing / George Murphy -- Direct sequencing of inserts cloned into lambda vectors / Afshan N. Malik -- Cosmid sequencing / Molly Craxton -- Genomic sequencing / Gerd P. Pfeifer and Arthur D. Riggs -- Sequencing of double-stranded PCR products / Susannah Gal -- Sequencing double-stranded linear DNA with sequenase and [[alpha]-³⁵S] dATP / Jean-Laurent Casanova -- Solid phase PCR sequencing of biotinylated products / Andrew Green and Mark Vaudin -- Cycle sequencing / Robert W. Blakesley -- Direct blotting electrophoresis / Stephan Beck -- Multiplex DNA sequencing / Stephan Beck -- DNA sequencing by chemiluminescent detection / Stephan Beck -- Reverse sequencing of M13 cloned DNA / Alan T. Bankier -- Terminal labeling of DNA for Maxam and Gilbert sequencing / Eran Pichersky -- DNA sequencing by the chemical method / Eran Pichersky -- DNA sequencing by chemical degradation using one, two, and four different fluorophores / André Rosenthal -- Linear amplification sequencing with dye terminators / André Rosenthal and D. Stephen Charnock-Jones -- Sequencing reactions for the Applied Biosystems 373A automated DNA sequencer / Nicolette Halloran, Zijin Du and Richard K. Wilson -- Sequencing reactions for ALF (EMBL) automated DNA sequencer / Wilhelm Ansorge [and others] -- Sequencing using the DuPont Genesis 2000 DNA analysis system / Len Hall -- Use of robotic workstations in DNA sequencing / Alan T. Bankier.

Includes bibliographical references and index.